April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Use Of Self-assembling Peptide As Artificial Vitreous In Rabbits
Author Affiliations & Notes
  • Yasutaka Hayashida
    Medical Ctr for Translational Research, Osaka University Hospital, Suita, Osaka, Japan
    Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
  • Koji Uesugi
    Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
    Menicon Co., Ltd., Kasugai, Aichi, Japan
  • Ryuhei Hayashi
    Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
  • Koichi Baba
    Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
  • Takeshi Soma
    Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
  • Motokazu Tsujikawa
    Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
  • Hidenori Yokoi
    Menicon Co., Ltd., Kasugai, Aichi, Japan
  • Kohji Nishida
    Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
  • Footnotes
    Commercial Relationships  Yasutaka Hayashida, Menicoin, Co., Ltd. (F); Koji Uesugi, Menicon, Co., Ltd. (E); Ryuhei Hayashi, None; Koichi Baba, None; Takeshi Soma, None; Motokazu Tsujikawa, None; Hidenori Yokoi, Menicon, Co., Ltd. (E); Kohji Nishida, Menicoin, Co., Ltd. (F)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 460. doi:
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      Yasutaka Hayashida, Koji Uesugi, Ryuhei Hayashi, Koichi Baba, Takeshi Soma, Motokazu Tsujikawa, Hidenori Yokoi, Kohji Nishida; Use Of Self-assembling Peptide As Artificial Vitreous In Rabbits. Invest. Ophthalmol. Vis. Sci. 2011;52(14):460.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate a self-assembling peptide nanofiber scaffold for a potential vitreous substitution rabbits.

Methods: : Fourteen male Japanese white rabbits underwent a 25 gauge pars plana vitrectomy (PPV) with performing posterior vitreous detachment in the right eye. Approximately 1.5 mL of the self-assembling gel was then injected into the vitreous cavity in ten eyes. Four eyes underwent injection of balanced salt solution (BSS) served as controls. Slit-lamp microscopy, funduscopy, electroretinography (ERG) and intraocular pressure (IOP) was examined in each eye pre- and post-operatively. 4 weeks after surgery, the rabbits were all sacrificed and all eyes were enucleated and examined further histological analysis.

Results: : The self-assembling peptide gel was successfully injected and showed translucent cavity throughout the postoperative period. Any abnormal findings including elevation of IOP were not observed. ERG recordings confirmed preservation of retinal function with showing normal data. Histological examination of the retinal tissue showed no abnormalities.

Conclusions: : Rabbit model provided that the self-assembling peptide gel was safely injected by using 25 gauge PPV system and this artificial vitreous. Promising preliminary results of the use of self-assembling peptide gel as a vitreous substitute were shown. However, further investigation are needed for the long-term safety and the efficacy of the tamponade property.

Keywords: vitreous substitutes • injection • electroretinography: non-clinical 
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