April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Retinoid Binding Properties of the Nucleotide Binding Domains of Human ABCA4 Protein
Author Affiliations & Notes
  • Esther E. Biswas-Fiss
    Bioscience Technologies, Thomas Jefferson University, Philadelphia, Pennsylvania
  • Malissa Ha
    Bioscience Technologies, Thomas Jefferson University, Philadelphia, Pennsylvania
    Molecular Biology, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey
  • Deepa Kurpad
    Molecular Biology, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey
  • Subhasis B. Biswas
    Molecular Biology, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey
  • Footnotes
    Commercial Relationships  Esther E. Biswas-Fiss, None; Malissa Ha, None; Deepa Kurpad, None; Subhasis B. Biswas, None
  • Footnotes
    Support  NIH-NEI Grant EY 013113
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 51. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Esther E. Biswas-Fiss, Malissa Ha, Deepa Kurpad, Subhasis B. Biswas; Retinoid Binding Properties of the Nucleotide Binding Domains of Human ABCA4 Protein. Invest. Ophthalmol. Vis. Sci. 2011;52(14):51.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : The retina-specific ABC transporter, ABCA4, is essential for the recycling of 11-cis-retinal utilized by opsin as part of the visual transduction cycle. Mutations in the ABCA4 gene are associated with a broad range of inherited macular degenerations including Stargardt disease (STGD), autosomal recessive cone rod dystrophy (arCRD) and fundus flavimaculatus (FFM). Previously we have described the retinal binding activity of the second extracellular domain (ECD2) of human ABCA4 protein (Biswas-Fiss, E. E. et al. (2010) J Biol Chem. 285(25):19372-83). In this study the retinoid binding properties of the nucleotide binding domains (NBDs) of ABCA4 transporter were investigated.

Methods: : Using recombinant polypeptides derived from the amino acid sequences of NBD1and NBD2 domains, we have determined the interaction affinities of retinoid binding to these two domains of ABCA4.

Results: : Fluorescence anisotropy and tryptophan emission spectroscopy demonstrated considerable differences in affinities between these two domains.

Conclusions: : These results support the hypothesis that the nucleotide domains of ABCA4 may play further roles in protein function in addition to that of nucleotide hydrolysis; furthermore Stargardt disease associated mutations may lead to significant changes in the retinoid binding affinity of these domains.

Keywords: protein structure/function • retina: distal (photoreceptors, horizontal cells, bipolar cells) • retinoids/retinoid binding proteins 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×