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Esther Yang, Byung J. Lee, Jin H. Kim, Kyu W. Kim, Young S. Yu, Jeong H. Kim; Autophagy-induced Regression Of Hyaloid Vessels In Early Ocular Development. Invest. Ophthalmol. Vis. Sci. 2011;52(14):515.
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To investigate whether hyaloids vessel regression is mediated by autophagy in early ocular development
Using developing mouse retina of postnatal 4, 8, 12, 16 and 28, histological examination with H&E staining was performed. In addition, to determine the apoptotic or autophagic processes in hyaloid vessel regression, immunohistochemistry for LC-3 and cleaved caspase-3 and electron microscopy were performed. With treatment of cobalt chloride to mimic hypoxic condition in human umbilical vein endothelial cells (HUVECs), Western blotting for procaspase-3, cleaved caspase-3 and LC-3 I/II was performed. Next, with treatment of an autophagy inducer, rapamycin, LC-3 conversion in HUVEC and hyaloids vessel regression in developing eye was determined to confirm the autophagy processes in hyaloidal vascular endothelial cells.
In developing retina, hyaloid vessel regression coincided with retinal vascular development was observed, when interestingly apoptotic and autophagic processes were simultaneously occurred. Moreover, in vascular endothelial cells under hypoxic condition, LC-3 conversion was detected with caspase-3 activation. Actually an autophagy inducer, rapamycin induced autophagy-mediated cell death of vascular endothelial cells in dose-dependent manner. Furthermore, the antopgy inducer, rapamycin significantly remove hyaloids vessels in early developing eye.
Our results suggest that hyaloid vessel regression could be mediated by autophagy in early ocular development.
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