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Pratap Challa, Anna H. Bordelon, Guorong Li, Catherine Bowes Rickman, David L. Epstein, Pedro Gonzalez; Laminin β1 upregulation in Pseudoexfoliation syndrome. Invest. Ophthalmol. Vis. Sci. 2011;52(14):72.
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© ARVO (1962-2015); The Authors (2016-present)
To identify genes differentially expressed in lens epithelial tissues from pseudoexfoliation (XFS) donors.
Lens capsules with epithelium were obtained from XFS donors with and without glaucoma and age-matched control donors without glaucoma. Tissues were fixed in RNA later and were linearly amplified with the Ovation Biotin RNA amplification and Labeling System. Resulting labeled cDNAs were individually hybridized to Affymetrix Human Genome U133 Plus 2.0 high density microarrays. Data analysis was performed using the GeneSpring Software 7.2. Significant differential expression of selected genes was validated by Quantitative real-time PCR in non-amplified RNA from a different set of XFS and control donors.
A relatively small number of genes were differentially expressed in the lens epithelium from XFS donors compared to controls. The differences that were detected included a significant upregulation of laminin β1 and downregulation of both ADAMTS-15 (a disintegrin and metalloproteinase with thrombospondin motif-15) and type VIII collagen α2.
Identificatiion and validation of differential expression of laminin β1 and and two extracellular metabolism genes implicate these genes as potential candidate genes in the pathogenesis of XFS syndrome. They may play a role in the formation and/or accumulation of the extracellular material seen in pseudoexfoliation deposits.
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