April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Laminin β1 upregulation in Pseudoexfoliation syndrome
Author Affiliations & Notes
  • Pratap Challa
    Dept of Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Anna H. Bordelon
    Dept of Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Guorong Li
    Dept of Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Catherine Bowes Rickman
    Dept of Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • David L. Epstein
    Dept of Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Pedro Gonzalez
    Dept of Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Footnotes
    Commercial Relationships  Pratap Challa, None; Anna H. Bordelon, None; Guorong Li, None; Catherine Bowes Rickman, None; David L. Epstein, None; Pedro Gonzalez, None
  • Footnotes
    Support  NIH K23 EY014019, EY016228, core grant P30 EY005722, Research to Prevent Blindness
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 72. doi:https://doi.org/
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      Pratap Challa, Anna H. Bordelon, Guorong Li, Catherine Bowes Rickman, David L. Epstein, Pedro Gonzalez; Laminin β1 upregulation in Pseudoexfoliation syndrome. Invest. Ophthalmol. Vis. Sci. 2011;52(14):72. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To identify genes differentially expressed in lens epithelial tissues from pseudoexfoliation (XFS) donors.

Methods: : Lens capsules with epithelium were obtained from XFS donors with and without glaucoma and age-matched control donors without glaucoma. Tissues were fixed in RNA later and were linearly amplified with the Ovation Biotin RNA amplification and Labeling System. Resulting labeled cDNAs were individually hybridized to Affymetrix Human Genome U133 Plus 2.0 high density microarrays. Data analysis was performed using the GeneSpring Software 7.2. Significant differential expression of selected genes was validated by Quantitative real-time PCR in non-amplified RNA from a different set of XFS and control donors.

Results: : A relatively small number of genes were differentially expressed in the lens epithelium from XFS donors compared to controls. The differences that were detected included a significant upregulation of laminin β1 and downregulation of both ADAMTS-15 (a disintegrin and metalloproteinase with thrombospondin motif-15) and type VIII collagen α2.

Conclusions: : Identificatiion and validation of differential expression of laminin β1 and and two extracellular metabolism genes implicate these genes as potential candidate genes in the pathogenesis of XFS syndrome. They may play a role in the formation and/or accumulation of the extracellular material seen in pseudoexfoliation deposits.

Keywords: genetics • gene microarray • gene/expression 
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