March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Potential Of Undifferentiated Cells Isolated From Human Periodontal Ligament To Generate Retinal Cells
Author Affiliations & Notes
  • Li Huang
    Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China
  • Yiqun GENG
    Joint Shantou International Eye Centre, Shantou, China
  • Jiajian LIANG
    Joint Shantou International Eye Centre, Shantou, China
  • Herman S CHEUNG
    Department of Biomedical Engineering, University of Miami, Miami, Florida
  • Wai-ming TSANG
    Oral Maxillofacial Surgery and Dentistry, North District Hospital, Hong Kong, China
  • XiaoWu YAO
    Second Affiliated Hospital, Shantou, China
  • Chi-Pui PANG
    Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China
  • Hin-Fai YAM
    Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China
  • Footnotes
    Commercial Relationships  Li Huang, None; Yiqun Geng, None; Jiajian Liang, None; Herman S Cheung, None; Wai-ming Tsang, None; XiaoWu Yao, None; Chi-Pui Pang, None; Hin-Fai Yam, None
  • Footnotes
    Support  Internal funding from DOVS, CUHK
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 1127. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Li Huang, Yiqun GENG, Jiajian LIANG, Herman S CHEUNG, Wai-ming TSANG, XiaoWu YAO, Chi-Pui PANG, Hin-Fai YAM; Potential Of Undifferentiated Cells Isolated From Human Periodontal Ligament To Generate Retinal Cells. Invest. Ophthalmol. Vis. Sci. 2012;53(14):1127. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : We investigated the differentiation of the undifferentiated cells isolated from neural crest-derived periodontal ligament (PDLUCs) into retinal neurons and whether it can be an alternative source for retinal repair and regeneration.

Methods: : PDL tissues were obtained from teeth collected from healthy donors, aged 13 to 30, by manual scrapping and digestion with collagenase type I/III. The dissociated cells were cultured in DMEM/F12 supplemented with 10% fetal bovine serum and 2% penicillin/streptomycin for investigation of the expression profiles by imunohistochemistry and RT-PCR. To imitate forebrain development, PDLUCs were exposed to a retinal determination condition, i.e., antagonism of Wnt and BMP signaling, with IGF-1 and bFGF. Their capacity to differentiate into retinal cells was evaluated by expressions of retinal-specific markers using quantitative PCR and confocal double immunofluorescence.

Results: : Cultured PDLUCs at passages 2 to 5 expressed the mesenchymal and neural crest markers CD90, CD44, p75/NGFR and Nestin, and the embryonic cell antigen SSEA-4. They also expressed mRNA transcripts for Oct4, Sox2, Nanog and c-Myc. Under retinal determination condition, PDLUCs yielded neurospheres within 24 hours with an age-dependent decrease of neurosphere formation. These neurospheres, when plated on matrigel-coated surface, formed neural rosettes and proliferated. On day 20, Pax6 and rhodopsin were increased up to 50 folds. Retina genes RX, DCX and Lhx2 were also significantly up-regulated. Similar observations were made in a parallel experiment using human H9 embryonic stem cells.

Conclusions: : Neural crest-derived PDLUCs residing in human tooth exhibited properties of cells at embryonic state and the potential to undergo retinal lineage differentiation. They could provide an easily accessible and promising source for retinal cell replacement.

Keywords: retinal degenerations: cell biology 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×