March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Regulation of Lens Differentiation by Sumoylation with SUMO1/2/3
Author Affiliations & Notes
  • David W. Li
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
    College of Life Sciences, Hunan Normal University, Changsha, China
  • Lili Gong
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Weike Ji
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Mi Deng
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Jinping Liu
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Peichao Chen
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
    College of Life Sciences, Hunan Normal University, Changsha, China
  • Wen-Feng Hu
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
    College of Life Sciences, Hunan Normal University, Changsha, China
  • Xiaohui Hu
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
    College of Life Sciences, Hunan Normal University, Changsha, China
  • Footnotes
    Commercial Relationships  David W. Li, None; Lili Gong, None; Weike Ji, None; Mi Deng, None; Jinping Liu, None; Peichao Chen, None; Wen-Feng Hu, None; Xiaohui Hu, None
  • Footnotes
    Support  EY018380
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 1305. doi:https://doi.org/
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    • Get Citation

      David W. Li, Lili Gong, Weike Ji, Mi Deng, Jinping Liu, Peichao Chen, Wen-Feng Hu, Xiaohui Hu; Regulation of Lens Differentiation by Sumoylation with SUMO1/2/3. Invest. Ophthalmol. Vis. Sci. 2012;53(14):1305. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Previous studies have shown that SUMO1/2/3-mediated modification of proteins plays an important role in cell differentiation. Whether SUMO1/2/3-mediated sumoylation is involved in regulation of lens differentiation remains to be investigated.

Methods: : Both in vitro and in vivo sumoylation reactions were conducted to determine if lens proteins could be sumoylated. The sumoylation status of various lens proteins were analyzed by western blot analysis and immunohistochemistry. The functions of individual SUMO factors were tested by overexpression and knockdown studies.

Results: : During mouse lens development, SUMO1/2/3 were differentially expressed at different embryonic stages. Various lens proteins were differentially sumoylated by SUMO1 and SUMO2/3. Sumoylation of various transcription factors including Pax-6, SP-1, c-Maf and Prox-1 are necessary for regulating lens differentiation.

Conclusions: : Sumoylation via SUMO1/2/3 plays an importation in lens differentiation

Keywords: cataract • differentiation • transcription factors 
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