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Koji Hirano, Kaoru Araki-Sasaki, Yasuhiro Osakabe, Keisuke Tanaka, Masahiko Kuroda, Hideki Fukuoka, Satoshi Kawasaki, Naoyuki Maeda, Shigeru Kinoshita; The Signification Of Lactoferrin In Secondary Corneal Amyloidosis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1096.
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Significance of lactoferrin in secondary corneal amyloidosis (SCA) is point at issue. We have previously reported Glu561Asp polymorphism in SCA and suggested that lactoferrin might be a precursor protein of SCA. However, lactoferrin in SCA was only proved by immunohistochemistry. Aim of this study is to confirm lactoferrin at SCA in definite method.
The corneal samples were excised from 6 cases with SCA and one case with GDLD for its surgical treatment. The tear sample and recombinant lactoferrin were served as control. The excised tissues were extracted by lysis buffer including collagenase or NP-40. The re-suspended extracts were electrophorased by SDS-PAGE, transferred and employed for western blotting with anti-lactoferrin antibody. Amyloid deposition and lactoferrin expression were also confirmed by immunohistochemistry.
The presence of lactoferrin was confirmed in SCA as well as GDLD and tear, in terms of western blotting.
This study is the first direct demonstration of lactoferrin expression at SCA.
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