April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
The Signification Of Lactoferrin In Secondary Corneal Amyloidosis
Koji Hirano; Kaoru Araki-Sasaki; Yasuhiro Osakabe; Keisuke Tanaka; Masahiko Kuroda; Hideki Fukuoka; Satoshi Kawasaki; Naoyuki Maeda; Shigeru Kinoshita
Author Affiliations & Notes
  • Koji Hirano
    Ophthal Ban Buntane Hosp, Fujita Health Univ Sch of Med, Nagoya, Japan
  • Kaoru Araki-Sasaki
    Ideta Eye Hospital, Kumamoto, Japan
  • Yasuhiro Osakabe
    Dept of Pathology, Tokyo Medical University, Tokyo, Japan
  • Keisuke Tanaka
    Nippi Incorporated, Tokyo, Japan
  • Masahiko Kuroda
    Dept of Pathology, Tokyo Medical University, Tokyo, Japan
  • Hideki Fukuoka
    Dept of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Satoshi Kawasaki
    Dept of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Naoyuki Maeda
    Dept of Ophthalmology, Osaka University Medical School, Osaka, Japan
  • Shigeru Kinoshita
    Dept of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Footnotes
    Commercial Relationships  Koji Hirano, None; Kaoru Araki-Sasaki, None; Yasuhiro Osakabe, None; Keisuke Tanaka, None; Masahiko Kuroda, None; Hideki Fukuoka, None; Satoshi Kawasaki, None; Naoyuki Maeda, None; Shigeru Kinoshita, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1096. doi:
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      Koji Hirano, Kaoru Araki-Sasaki, Yasuhiro Osakabe, Keisuke Tanaka, Masahiko Kuroda, Hideki Fukuoka, Satoshi Kawasaki, Naoyuki Maeda, Shigeru Kinoshita; The Signification Of Lactoferrin In Secondary Corneal Amyloidosis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1096.

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Abstract

Purpose: : Significance of lactoferrin in secondary corneal amyloidosis (SCA) is point at issue. We have previously reported Glu561Asp polymorphism in SCA and suggested that lactoferrin might be a precursor protein of SCA. However, lactoferrin in SCA was only proved by immunohistochemistry. Aim of this study is to confirm lactoferrin at SCA in definite method.

Methods: : The corneal samples were excised from 6 cases with SCA and one case with GDLD for its surgical treatment. The tear sample and recombinant lactoferrin were served as control. The excised tissues were extracted by lysis buffer including collagenase or NP-40. The re-suspended extracts were electrophorased by SDS-PAGE, transferred and employed for western blotting with anti-lactoferrin antibody. Amyloid deposition and lactoferrin expression were also confirmed by immunohistochemistry.

Results: : The presence of lactoferrin was confirmed in SCA as well as GDLD and tear, in terms of western blotting.

Conclusions: : This study is the first direct demonstration of lactoferrin expression at SCA.

Keywords: cornea: clinical science • cornea: epithelium 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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