April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Cytokines in Human Tears: Identification and Comparison of Levels in Patients with Acute Seasonal Allergic Conjunctivitis (SAC) and Infectious Conjunctivitis
Author Affiliations & Notes
  • Isabella Baumgartner
    Ophthalmology, Medical University of Vienna, Vienna, Austria
  • Sophie Biffl
    Institute for Specific Prophylaxis and Tropical Medicine, Medical Universtiy of Vienna, Vienna, Austria
  • Saskia Maca
    Ophthalmology, Medical University of Vienna, Vienna, Austria
  • Gerhard Zlabinger
    Institute for Specific Prophylaxis and Tropical Medicine, Medical Universtiy of Vienna, Vienna, Austria
  • Lasmiss Mejdoubi
    Institute for Specific Prophylaxis and Tropical Medicine, Medical Universtiy of Vienna, Vienna, Austria
  • Talin Barisani-Asenbauer
    Institute for Specific Prophylaxis and Tropical Medicine, Medical Universtiy of Vienna, Vienna, Austria
  • Footnotes
    Commercial Relationships  Isabella Baumgartner, None; Sophie Biffl, None; Saskia Maca, None; Gerhard Zlabinger, None; Lasmiss Mejdoubi, None; Talin Barisani-Asenbauer, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1138. doi:
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      Isabella Baumgartner, Sophie Biffl, Saskia Maca, Gerhard Zlabinger, Lasmiss Mejdoubi, Talin Barisani-Asenbauer; Cytokines in Human Tears: Identification and Comparison of Levels in Patients with Acute Seasonal Allergic Conjunctivitis (SAC) and Infectious Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1138.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To analyze the cytokine profile in tears of patients with seasonal allergic conjunctivitis and patients with infectious conjunctivitis.

Methods: : Tears from patients with seasonal allergic conjunctivitis (SAC) (n=21), acute non-allergic infectious conjunctivitis (n=14), and healthy volunteers (n=10) were collected via plastic micropipettes and analyzed for cytokines L-1ß, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), IL-13, IFN , MCP-1, RANTES and TNFα via the Beadlyte® Human Multi-Cytokine Detection System 4 and Luminex® multiplex cytokine profiling system. The study was independently reviewed and approved by the ethical board of the Medical University of Vienna, Austria.

Results: : Compared to healthy controls, subjects with SAC showed higher concentrations for CCL5, Eotaxin, IL1b, IL4,IL5,IL6,IL8,IL13,VEGF and lower concentrations for IL12p70, MCP-1,MIP1a . Subjects with non-allergic conjunctivitis produced higher levels of CCL5, IFNg, IL10, IL13, IL1b, IL4, IL5, IL6, IL8, MCP-1,MIP1a,TNFa and reduced levels of IL12p40,IL12p70,Il17,IL2,IL7,VEGF, when compared to healthy controls. Concentrations of CCL5,IFNg, IL13,IL2, IL4,IL7 and VEGF in tears of allergic and non-allergic patients differed to a significant degree.

Conclusions: : Multiplex bead-based assays are convenient for cytokine/chemokine detection in tears. Our data indicate different cytokine profiles for SAC, non-SAC subjects and healthy volunteers. Cytokine profiling from tears could help in the differential diagnosis of conjunctivitis of unknown etiology and in the understanding of ocular surface pathophysiology.

Keywords: cytokines/chemokines • conjunctivitis 
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