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Isabella Baumgartner, Sophie Biffl, Saskia Maca, Gerhard Zlabinger, Lasmiss Mejdoubi, Talin Barisani-Asenbauer; Cytokines in Human Tears: Identification and Comparison of Levels in Patients with Acute Seasonal Allergic Conjunctivitis (SAC) and Infectious Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1138.
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© ARVO (1962-2015); The Authors (2016-present)
To analyze the cytokine profile in tears of patients with seasonal allergic conjunctivitis and patients with infectious conjunctivitis.
Tears from patients with seasonal allergic conjunctivitis (SAC) (n=21), acute non-allergic infectious conjunctivitis (n=14), and healthy volunteers (n=10) were collected via plastic micropipettes and analyzed for cytokines L-1ß, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), IL-13, IFN , MCP-1, RANTES and TNFα via the Beadlyte® Human Multi-Cytokine Detection System 4 and Luminex® multiplex cytokine profiling system. The study was independently reviewed and approved by the ethical board of the Medical University of Vienna, Austria.
Compared to healthy controls, subjects with SAC showed higher concentrations for CCL5, Eotaxin, IL1b, IL4,IL5,IL6,IL8,IL13,VEGF and lower concentrations for IL12p70, MCP-1,MIP1a . Subjects with non-allergic conjunctivitis produced higher levels of CCL5, IFNg, IL10, IL13, IL1b, IL4, IL5, IL6, IL8, MCP-1,MIP1a,TNFa and reduced levels of IL12p40,IL12p70,Il17,IL2,IL7,VEGF, when compared to healthy controls. Concentrations of CCL5,IFNg, IL13,IL2, IL4,IL7 and VEGF in tears of allergic and non-allergic patients differed to a significant degree.
Multiplex bead-based assays are convenient for cytokine/chemokine detection in tears. Our data indicate different cytokine profiles for SAC, non-SAC subjects and healthy volunteers. Cytokine profiling from tears could help in the differential diagnosis of conjunctivitis of unknown etiology and in the understanding of ocular surface pathophysiology.
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