April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Neural Activity in the Inner Retina After Photocoagulation
Author Affiliations & Notes
  • Bryan W. Jones
    Ophthalmology, Moran Eye Center, Salt Lake City, Utah
  • Phil Huie
    Ophthalmology, Stanford University, Salt Lake City, California
  • Haimei Wang
    Ophthalmology, Moran Eye Center, Salt Lake City, Utah
  • Alexander Sher
    Santa Cruz Institute for Particle Physic, University of California, Santa Cruz, Santa Cruz, California
  • Robert E. Marc
    Ophthalmology, Moran Eye Center, Salt Lake City, Utah
  • Daniel Palanker
    Ophthalmology, Stanford University, Salt Lake City, California
  • Footnotes
    Commercial Relationships  Bryan W. Jones, None; Phil Huie, None; Haimei Wang, None; Alexander Sher, None; Robert E. Marc, Signature Immunologics (E); Daniel Palanker, Patent (P)
  • Footnotes
    Support  RPB CDA, Thome Foundation(BWJ), BWF CASI(AS), NIH EY02576, NIH EY015128, NSF 0941717, NIH EY014800 Vision Core(RM), RPB award to Moran Eye Center, NIH 5R01EY18608, Air Force OSR, Stanford Bio-X(DP)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1170. doi:
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    • Get Citation

      Bryan W. Jones, Phil Huie, Haimei Wang, Alexander Sher, Robert E. Marc, Daniel Palanker; Neural Activity in the Inner Retina After Photocoagulation. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1170.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Retinal photocoagulation is a common clinical intervention in many retinopathies. Though clinically effective, current laser therapies result in scotomas and scarring. We have demonstrated that during healing of small and light photocoagulation lesions, photoreceptors from adjacent areas migrate into the coagulated zone, restoring retinal continuity. This approach could allow for retinal laser therapy without the common detrimental size effects. Our goal was to assess the inner retina activity after photocoagulation with different levels of severity.

Methods: : Laser exposures of "barely visible" and "moderate" grades were applied to rabbit retina (20ms, 200µm). 2 days and 2 months after photocoagulation the eyes were vitrectomized, retina was incubated for 30 minutes in vivo with 10mM 1-amino-4-guanidobutane (AGB) while exposed to flickering light, allowing AGB to permeate activated cation channels (iGluR, mGlurR6). The eyes were then aldehyde fixed in-situ, embedded in plastic, sectioned and processed for computational molecular phenotyping (CMP).

Results: : In the burns of moderate grade the two-month-old lesions were only partially filled with migrated photoreceptors, leaving scotomata. In the barely visible lesions, retinal pigment epithelium and photoreceptors were selectively ablated, but anatomic and metabolic signatures revealed robust bipolar, amacrine, horizontal and ganglion cell populations. These lesions filled in with photoreceptors after 2 months Signaling of the neural retina within these lesions, as revealed through AGB probing, was reduced 2 days after photocoagulation but was restored to normal levels after 2 months.

Conclusions: : Optimizing the laser spot size, radiant exposure and pulse duration to target photoreceptors, while preserving inner retina allows the adjacent photoreceptors to shift and rewire to the local inner neurons. This procedure, while achieving its therapeutic goal of reducing metabolic load through reduction in the number of photoreceptors, may help avoid scarring, vision loss and other associated side effects of current photocoagulation protocols. Additionally, targeted coagulation of photoreceptors may represent an adjustable and reversible model of retinal degeneration and neural plasticity.

Keywords: retina • retinal connections, networks, circuitry • laser 

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