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Hungwon Tchah, Kyoung Sub Lee, Mi Hyun Cheon, Dong Ah Ko, Jae Yong Kim, Myoung joon Kim, Eun-soon Kim; The Effects Of Mitomycin, Rapamycin And Bevacizumab On Corneal Haze After Photorefractive Keratectomy. Invest. Ophthalmol. Vis. Sci. 2012;53(14):1470.
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To investigate the effects of mitomycin, rapamycin and bevacizumab on the corneal haze and the number of keratocytes after photorefractive keratectomy(PRK)
PRK(3mm optica zone, 80μm depth) was done in one eye of Sprague-Dawly rats(n=12). 0.02% mitomycin (M group,n=3), 2.5% bevacizumab (B group,n=3), 0.01% rapamycin (R group,n=3), and BSS (control group,n=3) were applied respectively for 2 minutes just after PRK. Then operated eyes were exposed to UV-B(100mj/cm2) light foror 2 minutes at 3 weeks after PRK. Corneal haze was assessed up to 12 weeks after PRK. H&E stain and TUNEL stain were done at 3,6,12 weeks
A single intraoperative application of bevacizumab and rapamycin immediately after PRK induced opacity and apoptosis of keratocytes.Corneal haze was less severe in treated groups compared with control group. Twelve weeks after PRK, bevacizumab and rapamycin reduced corneal haze , the number of keratocytes, apoptotic cells similar with MMC, even after UV-B irradiation.(Corneal haze score :1±0 in M group, 1±1.0 in B group, 1±1.0 in R group, and 1.8±0.8 in control group at postop 12 weeks.) Apoptosis of keratocyte was detected in all 4 groups but relatively large numbers of apoptotic cells were found only in control group even after 12 weeks. Three weeks after PRK, dying stromal cells showed cell shrinkage, and chromatin condensation was observed in all treated groups by electron microscopy. Twelve weeks after PRK, fewer keratocytes and inflammatory cells were observed just beneath the epithelial layer in Group M,B and R than control group.
Bevacizumab and rapamycin is a potent inhibitor of corneal haze induced by PRK. It seems that bevacizumab and rapamycin were less toxic than mitomycin.
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