March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Comparative Stress Strain Measurements Of Human Corneas After Transepithelial UV-A Induced Cross-linking: Impregnation With Iontophoresis, Different Riboflavin Solutions And Irradiance Power
Author Affiliations & Notes
  • Riccardo Vinciguerra
    Opthalmology, Istituto Clinico Humanitas, Milan, Italy
  • Eberhard Spoerl
    Ophthalmology, University of Dresden, Dresden, Germany
  • Mario R. Romano
    Opthalmology, Istituto Clinico Humanitas, Milan, Italy
  • Pietro Rosetta
    Opthalmology, Istituto Clinico Humanitas, Milan, Italy
  • Paolo Vinciguerra
    Opthalmology, Istituto Clinico Humanitas, Milan, Italy
  • Footnotes
    Commercial Relationships  Riccardo Vinciguerra, None; Eberhard Spoerl, None; Mario R. Romano, None; Pietro Rosetta, None; Paolo Vinciguerra, SOOFT (C)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 1518. doi:https://doi.org/
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      Riccardo Vinciguerra, Eberhard Spoerl, Mario R. Romano, Pietro Rosetta, Paolo Vinciguerra; Comparative Stress Strain Measurements Of Human Corneas After Transepithelial UV-A Induced Cross-linking: Impregnation With Iontophoresis, Different Riboflavin Solutions And Irradiance Power. Invest. Ophthalmol. Vis. Sci. 2012;53(14):1518. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To compare the change in biomechanical properties of human cadaver corneas after standard transepithelial cross-linking (CXL-TE) versus CXL-TE using iontophoresis, different solutions and UV-A power.

Methods: : Twelve human cadaver corneas were divided in 4 different groups according to methods of impregnations and UV-A power used: Group A (three corneas, treated with CXL-TE using an irradiance power of 3 mW/cm2 for 30 minutes and riboflavin solution with 15 % dextrane and Tromethamine); Group B (three corneas treated with CXL-TE using an irradiance power of 3 mW/cm2 for 30 minutes and riboflavin solution with Tromethamine); Group C (three corneas treated with CXL-TE using an irradiance power of 10 mW/cm2 for 10 minutes and riboflavin solution with Tromethamine); Group D (three corneas treated with an irradiance power of 10 mW/cm2 for 10 minutes, the impregnation was obtained with the aid of iontophoresis and a riboflavin solution with Tromethamine). After cross- linking, static stress-strain measurements of the corneas were performed using a microcomputer-controlled biomaterial tester with a pre-stress of 5x103 Pa. Stress stain curves were fitted with an exponential function and the Young's modulus was calculated. Thickness of the cornea was measured with an ultrasound pachymeter.

Results: : Stress strain measurement showed an increase in corneal rigidity after cross-linking compared to standard CXL-TE, indicated by a rise in strain and in Young’s modulus calculated at 10% strain. Considering group A as standard of comparison, group B showed an increase by a factor of 1.45, group C by a factor of 1.26, group D by a factor of 1.81. Mean corneal thickness was: 627 µm for group A, 628 µm for group B, 527 µm for group C, 665 µm for group D.

Conclusions: : CXL-TE is able to increase mechanical rigidity in human corneas in selected groups. Stress strain results showed a maximal effect in the iontophoresis group, probably due to the increased riboflavin concentration in the stroma. Stress strain measurement in the other groups showed a better results using riboflavin solution without dextrane and 3 mW/cm2 of irradiance power.

Keywords: cornea: basic science • keratoconus • clinical laboratory testing 
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