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Marie O. Pequignot, Karim Chekroud, Carl Arndt, Danièle Basset, Christian P. Hamel, Philippe Brabet; Simple And Efficient: Validation Of A Cotton Wick Electrode For Animal Electroretinography. Invest. Ophthalmol. Vis. Sci. 2011;52(14):696.
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© ARVO (1962-2015); The Authors (2016-present)
Fatp1-/- mice are deficient for the fatty acid transport protein 1, a protein that has been shown to negatively regulates RPE65 in vitro. To test whether or not the lack of Fatp1-/- affects photoreceptor function and visual pigment regeneration, we performed ERG testing. To improve the sensitivity of the test, it was necessary to optimize the ERG process.
We designed a modified electrode made of a cotton wick and impregnated with NaCl. We analyzed the ERG profiles of the Fatp1-/- mouse model obtained using the cotton-wick electrode in comparison to those obtained with a silver wire loop electrode.
The electroretinogram recordings obtained using the silver wire loop electrodes suggested that there was no difference in the ERG amplitudes of Fatp1+/+ and Fatp1-/- mice. In contrast, as the cotton-wick electrode gave higher amplitude recordings and a better reproducibility of the recordings over time, we were able to detect a statistically significant, 10% decrease in both a- and b-wave amplitudes in the Fatp1-/- mice compared to Fatp1+/+.
Cotton electrodes are easy to make and provides reliable ERG recordings of higher amplitudes and lower variability. Results obtained in Fatp1-/- mice suggest mild photoreceptor dysfunction, reminiscent of the dysfonction observed in animals lacking DHA. This is consistent with the role of FATP1 in the lipid transport.
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