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Evgeniya Gorbunova, Anzhelika Berezovskaya, Victor Gunter, Olga Krivosheina, Igor Zapuskalov; Intraorbital Biotechnological Implants of Porous Titanium Nickelide Impregnated with Autologous Blood Monocytes after Evisceroenucleation in Vistar Rats. Invest. Ophthalmol. Vis. Sci. 2011;52(14):730.
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© ARVO (1962-2015); The Authors (2016-present)
To study the regenerative course after intraorbital implantation of porous titanium nickelide (TN) impregnated with autologous blood monocytes (IITNM) after evisceroenucleation in Vistar rats.
Monolateral evisceroenucleation was performed in 12 adult Vistar rats: 6 subsequently received an IITNM, and the other 6 received plane intraorbital TN implant (sham). The implant was a 4-mm in diameter spherical coil of TN thread, either plane or treated with autologous monocytes fractioned preliminary on a density gradient. Animals were followed with biomicroscopy and photography on the 1, 3, 7, 14, and 21 days after surgery for 21 days. 3 IITNM animals were sacrificed on the 14th postoperative day. The rest 3 IITNM and all sham animals were sacrificed on the 21st postoperative day. Histological analysis of the intraorbital content was performed.
On the 14th day after surgery, the IITMN group showed massive formation of granulation tissue presented by multiple small chaotic collagen fibers rarely forming bundles, and generalized heavy lymphocytic infiltration. Growth of young new vessels was observed along the edge of the metal thread peripherally. Corneal stroma showed thickened twisted fibers with new vessels in between. On the 21st day, there was a formation of organized bundles of thick collagen fibers and localized lymphocytic infiltrates concentrated closer to the center of the implant. Multiple new vessels had thick walls and were profound in the periphery. Structures of the eyeball were undifferentiated. The sham group showed formation of granulations with chaotic bundles of thin fibers and rare random solitary new vessels. Corneal tissue was well-defined along with loci of muscles and fat.
Suggested biotechnological implant shows modest inflammation and strong fibrosis at 3-week postoperative period in Vistar rats. Our results may be promising for future clinical use in anophthalmic patients for successful reconstructive surgery anticipating persistence of the eye prosthesis with minimal risk of complications.
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