April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Assessment of Lacrimal Canalicular Elastic Fibre Phenotype in Normal Tissue
Author Affiliations & Notes
  • John Bladen
    Ophthalmology, Moorfields Eye Hospital, London, United Kingdom
  • Tahrina Salam
    Ophthalmology, Moorfields Eye Hospital, London, United Kingdom
  • Christine Gaughan
    UCL, Institute of Ophthalmology, London, United Kingdom
  • Dan Ezra
    NIHR Biomedical Research Centre, Moorfields and Institute of Ophthalmology, London, United Kingdom
  • Geoff Rose
    Ophthalmology, Moorfields Eye Hospital, London, United Kingdom
  • Footnotes
    Commercial Relationships  John Bladen, None; Tahrina Salam, None; Christine Gaughan, None; Dan Ezra, None; Geoff Rose, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 737. doi:
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      John Bladen, Tahrina Salam, Christine Gaughan, Dan Ezra, Geoff Rose; Assessment of Lacrimal Canalicular Elastic Fibre Phenotype in Normal Tissue. Invest. Ophthalmol. Vis. Sci. 2011;52(14):737.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : The pathogenesis of functional conditions affecting the lacrimal canaliculus is poorly understood. Little is known about the structural components of the extracellular matrix (ECM) supporting the canaliculus. We characterize the elastic fibre phenotype of pericanalicular ECM.

Methods: : This study was conducted with local and regional ethical approval. Healthy lower eyelid tissue containing the nasolacrimal canalicular system obtained from the clear margins of 8 patients who underwent orbital exenteration, and cut into 5µm thick histological sections. Elastic fibres were detected using standard tinctorial methods. Verhöeff’s iron haematoxylin was used to stain mature elastic fibres (MEF). Gomori’s aldehyde fuchsin (GAF) was applied to identify MEF and elaunin fibres. Lillie’s oxidised aldehyde fuchsin (OxAF) was used to detect additional oxytalan fibres that are not detected by the other staining methods. Light microscopy assessment by independent histopathologists using semiquantative techniques grading abundance of elastic fibres on a short ordinal scale of 0-4 was performed. Distribution of fibres was descriptive only.

Results: : All three staining methods showed dense staining of elastic fibres. Verhöeff’s showed rich collection of MEF (3.1) with an almost distinct layer within the ECM surrounding the epithelial basement membrane. OxAF (3.4) stained more fibres than GAF (3.0) indicating the presence of additional elaunin fibres. OxAF staining identified a denser mid-submucosal elastic fibre collection. In addition, the fibres closest to the epithelium basement membrane were well ordered in contrast more distant ECM areas where the elastic fibres were more loosely organised.

Conclusions: : Staining of normal canaliculi revealed an abundance of different elastic fibre phenotypes within the canalicular submucosal ECM. The high density of elastic fibres suggests its importance in maintaining the normal lacrimal pump mechanism. Further research identifying changes in these elastic fibres in functional canalicular disorders is warranted.

Keywords: pathology: human • extracellular matrix • anatomy 

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