April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Disruption Of Epithelial Cells In Ephrin-A5 Knockout Lenses
Author Affiliations & Notes
  • Catherine Cheng
    School of Optometry and Vision Science Program, University of California, Berkeley, Berkeley, California
  • Xiaohua Gong
    School of Optometry and Vision Science Program, University of California, Berkeley, Berkeley, California
  • Footnotes
    Commercial Relationships  Catherine Cheng, None; Xiaohua Gong, None
  • Footnotes
    Support  NEI Grant EY013849 (XG)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 810. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Catherine Cheng, Xiaohua Gong; Disruption Of Epithelial Cells In Ephrin-A5 Knockout Lenses. Invest. Ophthalmol. Vis. Sci. 2011;52(14):810.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : To investigate the mechanism for how disrupted of Eph-ephirn signaling leads to age-related cataracts in humans and mice, and to study the roles of Eph-ephrin in the lens during development by using ephrin-A5 knockout (-/-) mice.

Methods: : Lens phenotypes of ephrin-A5(-/-) mice were examined using histology, and confocal images of GFP-positive lens epithelial and fiber cells were collected. Cell-cell junctions, cell adhesions and cytoskeletal structures were examined by immunohistochemistry. Lens signaling and structure proteins were analyzed by biochemical methods.

Results: : Ephrin-A5(-/-) mice develop variable cataract phenotypes with incomplete penetrance. Morphological data reveal aberrant or mislocalized epithelial cells and selectively disorganized fiber cells in ephrin-A5(-/-) lenses. Images of GFP-positive ephrin-A5(-/-) lenses show central epithelial cells with irregular shape and/or size and small areas of disordered fiber cells. Three-dimensional reconstruction show epithelial cells dipped into the anterior suture in some ephrin-A5(-/-) lenses. Immunostaining reveal that ephrin-A5 proteins are highly expressed at the lateral surfaces of lens epithelial cells and the distribution of N-cadherin and beta-catenin proteins are disrupted in ephrin-A5(-/-) epithelial cells.

Conclusions: : The loss of ephrin-A5 causes obvious changes in lens epithelial cells, indicating that ephrin-A5 signaling is essential for maintaining the morphology, polarity and communication of epithelial cells. These changes in the epithelial cells are likely to be early events that lead to cataracts in ephrin-A5(-/-) lenses. Thus, variable cataract formation in ephrin-A5(-/-) lenses is at least in part due to interactions between altered epithelial cells and disorganized underlying fiber cells.

Keywords: cataract • development 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.