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Mizuki Kitamura, Shikun He, Takeshi Yoshida, Christine Spee, Stephen J. Ryan, David R. Hinton; αB-crystallin Expression is Regulated by Histone Deacetylase Inhibitor in Human Fetal Retinal Pigment Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2011;52(14):868.
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© ARVO (1962-2015); The Authors (2016-present)
αB-crystallin belongs to the small heat shock protein family and plays a role as a chaperon for VEGF in angiogenesis. The aim of the present study is to investigate whether the inhibition of histone deacetylation is involved in the regulation of αB-crystallin in human fetal retinal pigment epithelial (RPE) cells.
Early passage, confluent, cultured fetal human RPE cells were used for the study. RPE cells were switched to serum free medium for 16 h and were then exposed to histone deacetylase inhibitor, trichostatin A (TSA) at various concentrations (0.1 µM to 1.0 µM) for 24 h. αB-crystallin mRNA expression was determined by RT-PCR. The expression of αB-crystallin, acetylated histone H2B, acetylated histone H4 and GRP78 in cell lysate were examined by western blot.
The highest expression of αB-crystallin mRNA was at the 0.3 µM concentration of TSA. TSA significantly down-regulated the expression of αB-crystallin in TSA concentration dependent manner, however, TSA didn’t change the expression of acetylated histone H4 and GRP78. The expression of acetylated histone H2B was not detected.
The result shows that αB-crystallin is regulated by histone deacetylase inhibitor. Our data suggest that histone deacetylase inhibitor have potential as a therapeutic strategy.
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