April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Regulation of Retinal Pigment Epithelium Tight Junctions Following Laser Photocoagulation
Masahiko Sugimoto; Bela Anand-Apte
Author Affiliations & Notes
  • Masahiko Sugimoto
    Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Beachwood, Ohio
  • Bela Anand-Apte
    Ophthalmic Research, Cole Eye Institute, Cleveland Clinic, Beachwood, Ohio
  • Footnotes
    Commercial Relationships  Masahiko Sugimoto, None; Bela Anand-Apte, None
  • Footnotes
    Support  This work was supported in part by US National Institute of Health EY016490, CA106415, EY015638, unrestricted Grant from Research to Prevent Blindness and RPB Lew Wasserman award to BA-A.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 888. doi:
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      Masahiko Sugimoto, Bela Anand-Apte; Regulation of Retinal Pigment Epithelium Tight Junctions Following Laser Photocoagulation. Invest. Ophthalmol. Vis. Sci. 2011;52(14):888.

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Abstract

Purpose: : To examine the effect of laser photocoagulation (PC) on cell-cell junctions of retinal pigment epithelium (RPE) cells.

Methods: : Human RPE cells (ARPE-19) grown to confluence on glass coverslips were photocoagulated using a 532-nm diode laser (532nm, 0.1 seconds, 75µm, 150mW). All analyses were conducted at 0, 24, 48 and 72 hours following PC. Tight junction (TJ) integrity was evaluated by western blot analysis of cell lysates and immunofluorescence microscopy of intact cells for expression and localization of TJ proteins ZO-1 and occludin. The conditioned medium of cells was analyzed for secretion of various angiogenic factors following PC.

Results: : PC induced disruption of TJs occurred in a time-dependent fashion along with concomitant secretion of epidermal growth factor (EGF). PC-induced phosphorylation of EGFR was observed by western blot analysis of whole-cell lysates. EGF receptor specific inhibitor (AG1478) could prevent the TJ disruption following PC.

Conclusions: : TJ disruption in RPE cells following PC is mediated by EGF.

Keywords: cell adhesions/cell junctions • laser • retinal pigment epithelium 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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