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Florian Sennlaub, Sophie Lavalette, Serge Camelo, Nicole Keller, Clotilde Théry, William Raoul; Milk Fat Globule-epidermal Growth Factor-factor VIII (MFGE8) / Lactadherin Deficiency Does Not Alter Retinal Homeostasis And Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2011;52(14):954.
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© ARVO (1962-2015); The Authors (2016-present)
MFGE8/lactadherin has been shown to be implicated in outer segments (OS) phagocytosis and angiogenesis. Its deficiency might therefore lead to retinal degeneration and choroidal neovascularization, cardinal features of AMD. We here studied the effect of MFGE8 deficiency on retinal homeostasis with age and on choroidal neovascularization (CNV)
MFG8 expressing cells were identified in Mfge8+/- mice expressing beta-galactosidase under a MFGE8 promotor. Mfge8+/- and Mfge8-/- mice were analyzed at 16-18 months of age and 3-month-old mice were submitted to laser induced choroidal neovascularization. Eyes were studied by funduscopy, histology, electron microscopy, scanning electron microscpoy of vascular corrosion casts of the choroid and CNV was analyzed on FITC-Dextran perfused choroidal flatmounts
MFGE8 is strongly expressed in photoreceptors and retinal glial cells but not in RPE cells. 18-month-old Mfge8-/- mice did not differ from controls in terms of photoreceptor cell layers (histology) or choroidal architecture (vascular corrosion cast). Bruchs membrane was slightly but significantly thicker in Mfge8-/- mice as compared to controls. CNV was comparable in Mfge8-/- and Mfge8+/- mice
Despite of the described important role of MFG8 in OS phagocytosis and neovascularization, MFG8 deficiency has no effect on long term retinal homeostasis and CNV development
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