April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Milk Fat Globule-epidermal Growth Factor-factor VIII (MFGE8) / Lactadherin Deficiency Does Not Alter Retinal Homeostasis And Choroidal Neovascularization
Author Affiliations & Notes
  • Florian Sennlaub
    Centre de Recherche des Cordeliers, INSERM, UMRS 872, Univ Paris 5 / Paris 6, Paris, France
  • Sophie Lavalette
    Centre de Recherche des Cordeliers, INSERM, UMRS 872, Univ Paris 5 / Paris 6, Paris, France
  • Serge Camelo
    Centre de Recherche des Cordeliers, INSERM, UMRS 872, Univ Paris 5 / Paris 6, Paris, France
  • Nicole Keller
    Centre de Recherche des Cordeliers, INSERM, UMRS 872, Univ Paris 5 / Paris 6, Paris, France
  • Clotilde Théry
    Institut Curie Research Center, INSERM U932, Paris, France
  • William Raoul
    Centre de Recherche des Cordeliers, INSERM, UMRS 872, Univ Paris 5 / Paris 6, Paris, France
  • Footnotes
    Commercial Relationships  Florian Sennlaub, None; Sophie Lavalette, None; Serge Camelo, None; Nicole Keller, None; Clotilde Théry, None; William Raoul, None
  • Footnotes
    Support  INSERM, ANR "blanc" (AO5120DD), ANR "Maladies Neurologiques et Maladies Psychiatriques" (R08098DS), ANR "Genopath" (R09099DS), ERC starting grant (ERC-2007 St.G. 210345)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 954. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Florian Sennlaub, Sophie Lavalette, Serge Camelo, Nicole Keller, Clotilde Théry, William Raoul; Milk Fat Globule-epidermal Growth Factor-factor VIII (MFGE8) / Lactadherin Deficiency Does Not Alter Retinal Homeostasis And Choroidal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2011;52(14):954.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : MFGE8/lactadherin has been shown to be implicated in outer segments (OS) phagocytosis and angiogenesis. Its deficiency might therefore lead to retinal degeneration and choroidal neovascularization, cardinal features of AMD. We here studied the effect of MFGE8 deficiency on retinal homeostasis with age and on choroidal neovascularization (CNV)

Methods: : MFG8 expressing cells were identified in Mfge8+/- mice expressing beta-galactosidase under a MFGE8 promotor. Mfge8+/- and Mfge8-/- mice were analyzed at 16-18 months of age and 3-month-old mice were submitted to laser induced choroidal neovascularization. Eyes were studied by funduscopy, histology, electron microscopy, scanning electron microscpoy of vascular corrosion casts of the choroid and CNV was analyzed on FITC-Dextran perfused choroidal flatmounts

Results: : MFGE8 is strongly expressed in photoreceptors and retinal glial cells but not in RPE cells. 18-month-old Mfge8-/- mice did not differ from controls in terms of photoreceptor cell layers (histology) or choroidal architecture (vascular corrosion cast). Bruchs membrane was slightly but significantly thicker in Mfge8-/- mice as compared to controls. CNV was comparable in Mfge8-/- and Mfge8+/- mice

Conclusions: : Despite of the described important role of MFG8 in OS phagocytosis and neovascularization, MFG8 deficiency has no effect on long term retinal homeostasis and CNV development

Keywords: age-related macular degeneration • choroid: neovascularization • aging 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×