March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Effects Of The Absence Of both Complement Factor H And Apolipoprotein E On Retinal Morphology In Mice
Author Affiliations & Notes
  • Laura Garcia-Garcia
    Experimental Ophthalmology Lab, Universidad de Navarra, Pamplona, Spain
  • Patricia Fernandez-Robredo
    Experimental Ophthalmology Lab, Universidad de Navarra, Pamplona, Spain
  • Sergio Recalde
    Experimental Ophthalmology Lab, Universidad de Navarra, Pamplona, Spain
  • Maite Moreno-Orduña
    Experimental Ophthalmology Lab, Universidad de Navarra, Pamplona, Spain
  • Vanessa Fernandez-Garcia
    Experimental Ophthalmology Lab, Universidad de Navarra, Pamplona, Spain
  • Josemaria Caire
    Experimental Ophthalmology Lab, Universidad de Navarra, Pamplona, Spain
  • Miguel A. Marchena-Fernandez
    Physiology, University of Alcala, Alcala de Henares, Spain
  • Pedro de la Villa
    Physiology, University of Alcala, Alcala de Henares, Spain
  • Alfredo Garcia-Layana
    Experimental Ophthalmology Lab, Universidad de Navarra, Pamplona, Spain
    Ophthalmology, Clinica Universidad de Navarra, Pamplona, Spain
  • Footnotes
    Commercial Relationships  Laura Garcia-Garcia, None; Patricia Fernandez-Robredo, None; Sergio Recalde, None; Maite Moreno-Orduña, None; Vanessa Fernandez-Garcia, None; Josemaria Caire, None; Miguel A. Marchena-Fernandez, None; Pedro de la Villa, None; Alfredo Garcia-Layana, None
  • Footnotes
    Support  supported by RETICS RD07/0062
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 1644. doi:https://doi.org/
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      Laura Garcia-Garcia, Patricia Fernandez-Robredo, Sergio Recalde, Maite Moreno-Orduña, Vanessa Fernandez-Garcia, Josemaria Caire, Miguel A. Marchena-Fernandez, Pedro de la Villa, Alfredo Garcia-Layana; Effects Of The Absence Of both Complement Factor H And Apolipoprotein E On Retinal Morphology In Mice. Invest. Ophthalmol. Vis. Sci. 2012;53(14):1644. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Apolipoprotein E deficient mouse (apoE-/-), experimental model of genetic hypercholesterolemia, shows ultrastructural retinal alterations. Complement factor H (CFH) is involved in inflammatory process associated to retinal degenerations. We aimed to investigate the effect of the absence of CFH and apoE genes in mouse retinal pigment epithelium (RPE) and Bruch's Membrane (BM).

Methods: : Eight WT, 15 CFH-/-/apoE-/- (DKO) and 19 CFH+/-/apoE+/- (DH) mice older than 12 months were used (all mice were in C57BL6/J background). Light microscopy was performed to analyze the following morphological parameters in the retinal sections (Toluidine Blue). Apoptotic-like nuclei from the outer nuclear layer (ONL) were counted using AnalySIS software. The thickness of the whole retina and the outer nuclear layer section were measured by ImageJ image processing program. Samples were also processed for Transmission Electron Microscopy (TEM) visualization. For comparisons after a significant ANOVA among groups, post-hoc tests were applied (SPSS v.15.0).

Results: : Apoptotic-like nuclei were significantly higher (p<0.001) among WT and genetically modified groups (p<0.001) and between DKO and DH groups (p<0.05). However, no differences were found for ONL and total retina among groups. In the TEM visualization structural abnormalities were found in DKO mice compared to WT. BM appeared swelled and with vacuoles were shown inside the membrane. Moreover, basal laminar deposits were observed in genetically modified groups whereas in the RPE electrodense and electrolucent vacuoles, phagosomes and swelling of interdigitations could be found in genetically mice.

Conclusions: : These results show that the absence of CFH and apoE is involved in the development of RPE and BM morphological alterations and apoptosis of ONL cells. This animal model could be useful as a model of retinal degeneration.

Keywords: retina • Bruch's membrane 
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