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Ali R. Djalilian, Hossein M. Sagha, Asad Movahedan, Behrad Y. Milani, Mercede Majdi, Robert M. Lavker, Beatrice Yue; The effect of Notch Signaling on Wnt Pathway genes in Corneal Epithelial cells. Invest. Ophthalmol. Vis. Sci. 2012;53(14):1822.
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© ARVO (1962-2015); The Authors (2016-present)
Notch and Wnt signaling are highly conserved cell-determination pathways in many cell types. This study was undertaken to evaluate the interaction between Notch & Wnt signaling in the corneal epithelium.
Primary human corneal epithelial cells were isolated from limbal rings. After first passage the cells were exposed to a gamma secretase inhibitor to inhibit Notch signaling. Forty eight hours later, the cells were harvested & RNA was extracted & subjected to RT-PCR.
Human corneal epithelial cells in-vitro expressed a number of Wnt protein, including Wnt3, Wnt5a, and Wnt7a. Treatment with gamma secretase inhibitor signaling increased the expression of Wnt3, while expression of Wnt5a & Wnt7a was slightly decreased.
Inhibition of Notch signaling appears to affect the expression of Wnt pathway genes. Based on these preliminary results, the effects of Notch on canonical & non-canonical Wnts appear to be different. Further studies are undergoing to determine the effect of Notch inhibition on other Wnt pathway genes.
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