Abstract
Purpose: :
IN THIS STUDY, WE AIMED TO INVESTIGATE THE THERAPEUTIC EFFECTS OF PROANTHOCYANIDIN (PAC) AND CO Q-10 ON MECHLORETHAMINE (MEC) INDUCED OCULAR SURFACE INJURY.
Methods: :
A TOTAL OF 18 MALE SPRAQUE-DAWLEY RATS WERE DIVIDED INTO FOUR GROUPS: CONTROL, MEC, MEC+PAC AND MEC+Co Q-10. THREE GROUPS RECEIVED SINGLE DOSE OF MEC (0.1 MG/5µL) DISTILLED ON THE OCULAR SURFACE. CONTROL ANIMALS WERE GIVEN SALINE ONLY.PAC (100 MG/KG) WAS ADMINISTERED BY GAVAGE FEEDING AND Co Q-10 (10 MG/KG) INTRAPERITONEALLY 30 MIN AFTER THE APPLICATION OF MEC, AND AFTER THE SAME DOSES OF PAC AND Co Q-10 WAS GIVEN; EVERY DAY FOR A TOTAL OF FIVE DOSES.
Results: :
MEC APPLICATION RESULTED IN SEVERE HISTOPATHOLOGICAL CHANGES. PAC SHOWED CONSIDERABLE PROTECTION AGAINST OCULAR DAMAGE. PATHOLOGIC CHANGES LIKE CORNEAL EPITHELIAL DEFECTS, STROMAL EDEMA AND NEOVASCULARIZATION, LIMBAL LYMPHOCYTE INFILTRATION, DESTRUCTION OF THE MUSCLE FIBERS AND IN THE LID, DEFORMATION OF THE HAIR FOLLICLES ON THE EYELASHES AND LOSS OF HYPODERMAL CONNECTIVE TISSUE FIBERS WERE ALMOST ABSENT. ON THE OTHER HAND; PROTECTIVE EFFECTS OF Co Q-10 FOUND LIMITED WHEN COMPARED TO PAC
Conclusions: :
OXIDATIVE STRESS COULD HAVE AN IMPORTANT ROLE IN THE MEC INDUCED OCULAR DAMAGE AS WELL AS INFLAMMATION. BEING ONE OF THE MOST EFFECTIVE ANTI-OXIDANTS, PAC AND CO Q-10 CAN BE A VALUABLE ADJUNCT IN FIGHTING THE ACUTE EFFECTS OF MEC INDUCED OCULAR INJURIES IN COURSE OF A CHEMICAL WARFARE.
Keywords: trauma • ocular irritants • wound healing