April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Increased Expression of Periostin in Vitreous and Fibrovascular Membranes from Patients with Proliferative Diabetic Retinopathy
Author Affiliations & Notes
  • Shigeo Yoshida
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Keijiro Ishikawa
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Ryo Asato
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
    Ophthalmology, Fukuoka University Chikushi Hospital, Chikushino-shi, Japan
  • Yukio Sassa
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
    Ophthalmology, Fukuoka University Chikushi Hospital, Chikushino-shi, Japan
  • Hiroshi Yoshikawa
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Kenji Izuhara
    Laboratory Medicine, Saga Medical School, Saga-shi, Japan
  • Toshihiro Kono
    Ophthalmology, Fukuoka University Chikushi Hospital, Chikushino-shi, Japan
  • Tatsuro Ishibashi
    Ophthalmology, Kyushu University, Fukuoka-shi, Japan
  • Footnotes
    Commercial Relationships  Shigeo Yoshida, None; Keijiro Ishikawa, None; Ryo Asato, None; Yukio Sassa, None; Hiroshi Yoshikawa, None; Kenji Izuhara, None; Toshihiro Kono, None; Tatsuro Ishibashi, None
  • Footnotes
    Support  the Ministry of Education, Science, Sports and Culture, Japan
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1268. doi:
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      Shigeo Yoshida, Keijiro Ishikawa, Ryo Asato, Yukio Sassa, Hiroshi Yoshikawa, Kenji Izuhara, Toshihiro Kono, Tatsuro Ishibashi; Increased Expression of Periostin in Vitreous and Fibrovascular Membranes from Patients with Proliferative Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1268.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Preretinal fibrovascular membranes (FVMs) form as a sequela to proliferative diabetic retinopathy (PDR), and their presence can lead to severe decrease of vision. The purpose of this study was to determine whether periostin, a matricellular protein that plays a role in cell adhesion and migration, is associated with the formation of FVMs.

Methods: : One hundred and six vitreous samples and 15 FVMs were obtained during vitrectomy on patients with PDR. Semiquantitative RT-PCR was performed to determine the level of the mRNA expression of periostin. Immunohistochemical analyses were performed to determine the sites of the periostin expression in the FVMs. ELISA was used to measure the concentrations of periostin and vascular endothelial growth factor (VEGF) in the vitreous.

Results: : The level of the mRNA of periostin was high in 10 of 10 FVMs tested, but barely detectable in the control retinas. Sequencing of the periostin PCR products revealed three splice variants of the FVMs. Immunohistochemical analysis showed co-localization of periostin and α-SMA in cells of the FVMs. The concentration of periostin in the vitreous was significantly higher in the patients with PDR than in the 31 eyes with a macular hole or with an epiretinal membrane (P <0.001). Among the PDR patients, the mean vitreous level of periostin in eyes with FVMs was significantly higher than in those without FVMs (epicenter only; P <0.001). The correlation between the vitreous concentrations of periostin and VEGF was not significant.

Conclusions: : Our findings indicate that periostin may be involved in the development of FVMs.

Keywords: diabetic retinopathy • vitreous • vitreoretinal surgery 
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