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Shigeo Yoshida, Keijiro Ishikawa, Ryo Asato, Yukio Sassa, Hiroshi Yoshikawa, Kenji Izuhara, Toshihiro Kono, Tatsuro Ishibashi; Increased Expression of Periostin in Vitreous and Fibrovascular Membranes from Patients with Proliferative Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1268.
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Preretinal fibrovascular membranes (FVMs) form as a sequela to proliferative diabetic retinopathy (PDR), and their presence can lead to severe decrease of vision. The purpose of this study was to determine whether periostin, a matricellular protein that plays a role in cell adhesion and migration, is associated with the formation of FVMs.
One hundred and six vitreous samples and 15 FVMs were obtained during vitrectomy on patients with PDR. Semiquantitative RT-PCR was performed to determine the level of the mRNA expression of periostin. Immunohistochemical analyses were performed to determine the sites of the periostin expression in the FVMs. ELISA was used to measure the concentrations of periostin and vascular endothelial growth factor (VEGF) in the vitreous.
The level of the mRNA of periostin was high in 10 of 10 FVMs tested, but barely detectable in the control retinas. Sequencing of the periostin PCR products revealed three splice variants of the FVMs. Immunohistochemical analysis showed co-localization of periostin and α-SMA in cells of the FVMs. The concentration of periostin in the vitreous was significantly higher in the patients with PDR than in the 31 eyes with a macular hole or with an epiretinal membrane (P <0.001). Among the PDR patients, the mean vitreous level of periostin in eyes with FVMs was significantly higher than in those without FVMs (epicenter only; P <0.001). The correlation between the vitreous concentrations of periostin and VEGF was not significant.
Our findings indicate that periostin may be involved in the development of FVMs.
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