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Yukio Sassa, Shigeo Yoshida, Ryo Asato, Keijiro Ishikawa, Toshihiro Kono, Tatsuro Ishibashi, IV; The Concentration Changes Of Vegf And Mcp-1 In The Eyes Of The Patients With Proliferative Diabetic Retinopathy. (the Second Vitrectomy Cases). Invest. Ophthalmol. Vis. Sci. 2011;52(14):1269.
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To examine concentration changes of VEGF and MCP-1 in the vitreous obtained in the first vitrectomy compared to those in the second vitrectomy.
Vitreous fluid was obtained from 145 individuals undergoing pars plana vitrectomy at Fukuoka University Chikushi Hospital (from April 2007 to March 2010) in accordance with Human Discarded Specimen Research Protocol approved by institutional review boards. Vitreous fluid was obtained again from 10 individuals (10 eyes) undergoing the second pars plana vitrectomy because of prolonged activity of the diabetic retinopathy. Undiluted samples were collected at the time of surgery, immediately stored at -80 °C until used. The concentration of VEGF and MCP-1 were determined with ELISA method at the same time. In each case, other treatments including triamcinolone acetonide and/or anti-VEGF antibody were not performed.
The concentrations of VEGF did not decrease at the time of the second vitrectomy (average 1.23ng/ml) compared to those (average 1.12ng/ml) at the first vitrectomy (p=0.87). The concentration of MCP-1 significantly increase at the time of the second vitrectomy (average 2.68ng/ml) compared to those (average 0.61ng/ml) at the first vitrectomy (p=0.015). The reasons for the second vitrectomy were tractional retinal detachment (4eyes), neovascular glaucoma (NVG) (3eyes), persistent vitreous hemorrhage (2eyes) and macular packer (1eye). MCP-1 increased 2 times at the concentration in the tractional retinal detachment cases (4eyes) compared to the other 6 eyes and VEGF increases 6 times at the concentration in the NVG (3eyes) compared to the other 7eyes.
VEGF and MCP-1 may contribute to prolong the activity of the diabetic retinopathy. VEGF may develop NVG and MCP-1 may cause tractional retinal detachment.
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