Purchase this article with an account.
Wenhan Yu, Haotian Xiang, Guo Liu, Iok-Hou Pang, Jun Chen, Xuyang Liu; Ultrasound-Microbubble Mediated CNP Gene Transfer Lowered IOP in Rabbits and Monkeys. Invest. Ophthalmol. Vis. Sci. 2012;53(14):1930.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To evaluate C-type natriuretic peptide (CNP) gene expression delivered via ultrasound-microbubble mediated gene transfection and its effects on rabbit and monkey intraocular pressure (IOP).
Mixture (80 µl), containing plasmids (150 ng/µl) encoding CNP or copGFP dissolved in SonoVue® microbubble, was injected into the anterior chamberof New Zealand white rabbits or Tibetan monkeys after equal volume of aqueous humor was drawn. The limbus corneae was then exposed to ultrasound for 1 min (Frequency = 1 MHz, Intensity = 1 W/cm2, Duty Cycle = 20%) . IOP was monitored daily for 2 weeks by the TonoLab rebound tonometer. Frozen sections for rabbit limbus corneaes were prepared to confirm gene expression.
CNP gene transfer significantly (P<0.05) lowered rabbit and monkey IOP starting 48 h after treatment. In rabbits, the maximum reduction (1.85±0.51 mm Hg) was shown on day 5. In monkeys, the maximum reduction (3.17±0.95 mm Hg) was observed on day 3. The rabbit IOP change correlated with gene expression at the angle of anterior chamber, as well as the vicinal cornea and sclera.
Ultrasound-microbubble mediated transfection of CNP gene lowered IOP and may be a safe and effective means for glaucoma gene therapy.
This PDF is available to Subscribers Only