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Dana L. Holve, Glenwood Gum, Ricardo de Carvalho; Natural Course of Chorioretinitis Induced by Subretinal Injection to Form Acute (Escherichia Coli 0113:H10 Lipopolysaccharide) and Prolonged (Heat-Killed Mycobacterium Butyricum) Models. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1357.
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Evaluation of the efficacy of a chorioretinitis model induced by subretinal injection of lipopolysaccharide(LPS) or M. butyricum suspension.
New Zealand White rabbits(n=17) were randomly assigned to a control group challenged by a subretinal injection of hyaluronic acid(HA), one group challenged by subretinal injection of Escherichia coli 0113:H10 LPS in HA with a final concentration of 0.8 mg/ml and 400 ug/g of LPS with respect to HA, and three experimental groups challenged by subretinal injection of M. butyricum suspended in HA for a final concentration of 1.0 mg/ml, and sensitized by subcutaneous injection as follows: 1) rabbit receiving two 0.25 ml antigen/IFA, 2) rabbit receiving two 0.5 ml antigen/IFA, and 3) non-sensitized rabbits. Sham and LPS subretinal injection rabbits were challenged on Day 1; heat-killed M. butyricum rabbits were sensitized on Day 1, and challenged on Day 4. Subretinal injection consisted of ~ 20 µl of LPS, M. butyricum or sham suspension adjacent to optic nerve. Ophthalmic examination, color fundus photography and fluorescein angiography (FA) were evaluated over 30-days. Eyes were evaluated histopathologically.
Sham group demonstrated no evidence of chorioretinitis. Group challenged with LPS suspension showed acute panuveitis (Day 2); clinical findings consisted of moderate-to-severe cellular/aqueous flare in the anterior chamber initially. Severe cellular response of the posterior chamber progressed to mildly involve the anterior vitreous accompanied by a snow ball like formation in the inferior vitreous (up to 10 days) making the subretinal injection difficult to visualize. FA findings correlated with ophthalmic findings of moderate-to-severe choroid/retina inflammation and retinal vasculitis. All groups (sensitized or not) challenged by M. butyricum suspension demonstrated prolonged period (~30 days) of chorioretinitis with minimal involvement of the anterior chamber. Ocular exams and fundus photography revealed mild-to-moderate choroid/retinal inflammation, retinal hemorrhage and vitreal opacities. FA demonstrated vasculature leakage. Histopathology indicated granulomatous inflammation in LPS and M. butyricum groups.
Subretinal injection of LPS provides an acute model of chorioretinitis with initial anterior chamber involvement. Subretinal injection of M. butyricum is a good model for prolonged chorioretinitis with minimal involvement of the anterior chamber.
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