April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
The Ethanol Extract of Phyllostachys nigra Protects the Rat Retina against NMDA Induced Excitotoxocity
Author Affiliations & Notes
  • Kui Dong Kang
    Department of Ophthalmology, The Catholic University of Korea, Seoul, Republic of Korea
  • Kyung-A Kim
    Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Republic of Korea
  • Chul Young Kim
    Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Republic of Korea
  • Byung Hun Um
    Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Republic of Korea
  • Hyuong Jo
    Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Republic of Korea
  • Hong Ryul Ahn
    Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Republic of Korea
  • Jee Hyun Kwag
    Department of Brain and Cognitive Engineering, Korea University, Seoul, Republic of Korea
  • Aman Shah Bin Abdul Majid
    Universiti Sains Malaysia, Penang, Malaysia
  • Sang Hoon Jung
    Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Republic of Korea
  • Footnotes
    Commercial Relationships  Kui Dong Kang, None; Kyung-A Kim, None; Chul Young Kim, None; Byung Hun Um, None; Hyuong Jo, None; Hong Ryul Ahn, None; Jee Hyun Kwag, None; Aman Shah Bin Abdul Majid, None; Sang Hoon Jung, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1386. doi:
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      Kui Dong Kang, Kyung-A Kim, Chul Young Kim, Byung Hun Um, Hyuong Jo, Hong Ryul Ahn, Jee Hyun Kwag, Aman Shah Bin Abdul Majid, Sang Hoon Jung; The Ethanol Extract of Phyllostachys nigra Protects the Rat Retina against NMDA Induced Excitotoxocity. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1386.

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Abstract

Purpose: : The purpose of this study was to determine whether the ethanol extract of Phyllostachys nigra could attenuate N-methyl-D-aspartate (NMDA) induced excitotoxicity of the adult rat retina.

Methods: : Excitotoxicity was induced by intravitreal injection of NMDA and different concentration of the ethanol extract of Phyllostachys nigra was injected immediately after the insult. The inner retinal thickness (IRT) and TdT-mediated biotin-dUTP nick end labeling (TUNEL) positive cell counts in the retinal ganglion cell layer were evaluated to quantify the effect of the sample. Western blot analysis was performed to investigate the effect of the sample in the expression of various apoptotic proteins.

Results: : Intravitreal injection of NMDA induced apoptosis in the rat retina, which involved the cleavage of PARP-1 and caspase-3. The ethanol extract of Phyllostachys nigra significantly attenuated the negative effect of NMDA induced excitotoxicity both demonstrated by immunohistochemistry and Western blot analysis. Importantly, the ethanol extract of Phyllostachys nigra significantly increased the expression of catalase and SOD (superoxide dismutase).

Conclusions: : NMDA induced excitotoxicity was significantly blunted by the ethanol extract of Phyllostachys nigra which suggest that this compound may potentially be used as a neuroprotectant.

Keywords: neuroprotection • excitatory neurotransmitters 
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