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Kui Dong Kang, Kyung-A Kim, Chul Young Kim, Byung Hun Um, Hyuong Jo, Hong Ryul Ahn, Jee Hyun Kwag, Aman Shah Bin Abdul Majid, Sang Hoon Jung; The Ethanol Extract of Phyllostachys nigra Protects the Rat Retina against NMDA Induced Excitotoxocity. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1386.
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The purpose of this study was to determine whether the ethanol extract of Phyllostachys nigra could attenuate N-methyl-D-aspartate (NMDA) induced excitotoxicity of the adult rat retina.
Excitotoxicity was induced by intravitreal injection of NMDA and different concentration of the ethanol extract of Phyllostachys nigra was injected immediately after the insult. The inner retinal thickness (IRT) and TdT-mediated biotin-dUTP nick end labeling (TUNEL) positive cell counts in the retinal ganglion cell layer were evaluated to quantify the effect of the sample. Western blot analysis was performed to investigate the effect of the sample in the expression of various apoptotic proteins.
Intravitreal injection of NMDA induced apoptosis in the rat retina, which involved the cleavage of PARP-1 and caspase-3. The ethanol extract of Phyllostachys nigra significantly attenuated the negative effect of NMDA induced excitotoxicity both demonstrated by immunohistochemistry and Western blot analysis. Importantly, the ethanol extract of Phyllostachys nigra significantly increased the expression of catalase and SOD (superoxide dismutase).
NMDA induced excitotoxicity was significantly blunted by the ethanol extract of Phyllostachys nigra which suggest that this compound may potentially be used as a neuroprotectant.
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