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Emily S. Birkholz, Jessica M. Skeie, Michael E. Wright, Eric N. Brown, James C. Folk, Stephen R. Russell, H Culver Boldt, Karen M. Gehrs, Edwin M. Stone, Vinit B. Mahajan; Proteomic Analysis of Vitreous Biopsy Techniques. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1391.
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© ARVO (1962-2015); The Authors (2016-present)
To compare vitreous biopsy methods on analysis platforms used in proteomics biomarker discovery.
Simultaneous vitreous biopsy was performed with a needle and vitreous cutter instrument in 10 eyes graded as low, medium, or high viscosity. Vitreous specimens underwent comparative assays for total protein quantification, one-dimensional gel electrophoresis, and liquid chromatography with mass-spectrometry.
The total protein concentration obtained with a needle and vitrector biopsy measured of 1.10 mg/ml +/- 0.36 (SEM) and 1.13 mg/ml +/- 0.25 (SEM), respectively, with a very high correlation coefficient of 0.94 in eight eyes with low to medium viscosity. The correlation coefficient was reduced to 0.74 when two eyes with high viscosity vitreous were included. In one-dimensional gel electrophoresis, protein profiles appeared similar for each technique, however there was mild to moderate single band intensity variance.
Vitreous proteins collected from needle biopsies are similar to those obtained from a vitreous cutter instrument. This suggests vitreous samples from both techniques may be used in proteomics and biomarker discovery studies.
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