April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Proteomic Analysis of Vitreous Biopsy Techniques
Emily S. Birkholz; Jessica M. Skeie; Michael E. Wright; Eric N. Brown; James C. Folk; Stephen R. Russell; H Culver Boldt; Karen M. Gehrs; Edwin M. Stone; Vinit B. Mahajan
Author Affiliations & Notes
  • Emily S. Birkholz
    Ophthalmology, College of Medicine,
    University of Iowa Hospitals and Clinics, Iowa City, Iowa
  • Jessica M. Skeie
    Ophthalmology and Visual Sciences, Ophthalmology,
    University of Iowa Hospitals and Clinics, Iowa City, Iowa
  • Michael E. Wright
    Physiology,
    University of Iowa Hospitals and Clinics, Iowa City, Iowa
  • Eric N. Brown
    Ophthalmology, College of Medicine,
    University of Iowa, Iowa City, Iowa
  • James C. Folk
    Ophthalmology, College of Medicine,
    University of Iowa Hospitals and Clinics, Iowa City, Iowa
  • Stephen R. Russell
    Ophthalmology, College of Medicine,
    University of Iowa Hospitals and Clinics, Iowa City, Iowa
  • H Culver Boldt
    Ophthalmology, College of Medicine,
    University of Iowa Hospitals and Clinics, Iowa City, Iowa
  • Karen M. Gehrs
    Ophthalmology, College of Medicine,
    University of Iowa Hospitals and Clinics, Iowa City, Iowa
  • Edwin M. Stone
    Ophthalmology and Visual Sciences, Carver College of Medicine, Iowa City, Iowa
  • Vinit B. Mahajan
    Ophthalmology and Visual Sciences, Ophthalmology,
    University of Iowa, Iowa City, Iowa
  • Footnotes
    Commercial Relationships  Emily S. Birkholz, None; Jessica M. Skeie, None; Michael E. Wright, None; Eric N. Brown, None; James C. Folk, None; Stephen R. Russell, None; H Culver Boldt, None; Karen M. Gehrs, None; Edwin M. Stone, None; Vinit B. Mahajan, None
  • Footnotes
    Support  Unrestricted grant from Research to Prevent Blindness, Inc., New York, NY.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1391. doi:
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      Emily S. Birkholz, Jessica M. Skeie, Michael E. Wright, Eric N. Brown, James C. Folk, Stephen R. Russell, H Culver Boldt, Karen M. Gehrs, Edwin M. Stone, Vinit B. Mahajan; Proteomic Analysis of Vitreous Biopsy Techniques. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1391.

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Abstract

Purpose: : To compare vitreous biopsy methods on analysis platforms used in proteomics biomarker discovery.

Methods: : Simultaneous vitreous biopsy was performed with a needle and vitreous cutter instrument in 10 eyes graded as low, medium, or high viscosity. Vitreous specimens underwent comparative assays for total protein quantification, one-dimensional gel electrophoresis, and liquid chromatography with mass-spectrometry.

Results: : The total protein concentration obtained with a needle and vitrector biopsy measured of 1.10 mg/ml +/- 0.36 (SEM) and 1.13 mg/ml +/- 0.25 (SEM), respectively, with a very high correlation coefficient of 0.94 in eight eyes with low to medium viscosity. The correlation coefficient was reduced to 0.74 when two eyes with high viscosity vitreous were included. In one-dimensional gel electrophoresis, protein profiles appeared similar for each technique, however there was mild to moderate single band intensity variance.

Conclusions: : Vitreous proteins collected from needle biopsies are similar to those obtained from a vitreous cutter instrument. This suggests vitreous samples from both techniques may be used in proteomics and biomarker discovery studies.

Keywords: vitreous • proteomics • protein purification and characterization 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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