April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
The Use of Magnetic Nanoparticles to Enhance Ocular Gene Delivery
Vishal R. Chauhan; Suber S. Huang
Author Affiliations & Notes
  • Vishal R. Chauhan
    Department of Ophthalmology and Visual Sciences, Case Western Reserve University, University Hospitals Eye Institute University Hospitals Case Medical Center, Cleveland, Ohio
  • Suber S. Huang
    Department of Ophthalmology and Visual Sciences, Case Western Reserve University, University Hospitals Eye Institute University Hospitals Case Medical Center, Cleveland, Ohio
  • Footnotes
    Commercial Relationships  Vishal R. Chauhan, None; Suber S. Huang, American Academy of Family Physicians (C), American Academy of Ophthalmology (C), American Retina Foundation (C), Bausch & Lomb (C), Diabetic Retinopathy Clinical Research Network (DRCR) (C), Digital Healthcare, Inc (I), Digital Healthcare, Inc. (C), i2i Innovative Ideas, Inc. (I), Luminae, LLC (I), Merck & Co. Inc. (C), NEHEP/NEI/NIH (C), Philip F. and Elizabeth G. Searle – Suber Huang Chair Professorship (E), REDIARC (Retinal Diseases Image Analysis Reading Center studies)-Neurotech, Lux Bio, Pfizer, VRT (Vitreo Retinal Technologies), Alcon, Shering Plough, MacuSight (C), Retinal Dis Image Analysis Reading Center/Case (C), Second Sight (C), SurModics, Inc (C), SurModics, Inc. (I), Therapeutic Nanoparticle and Molecular Imaging (C), University Hospitals Eye Institute (E)
  • Footnotes
    Support  Philip F. and Elizabeth G. Searle - Suber Huang MD Endowed Chair
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1419. doi:
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      Vishal R. Chauhan, Suber S. Huang; The Use of Magnetic Nanoparticles to Enhance Ocular Gene Delivery. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1419.

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Abstract

Purpose: : To study the efficiency of gene transfer using magnetic nanoparticles in ARPE-19 cells.

Methods: : Green-fluorescence protein (GFP) plasmids were associated with paramagnetic nanoparticles (purchased from Chemicell). Gene delivery was then targeted to cultured human diploid retinal pigment epithelial (ARPE-19) cells by application of a magnetic field. Cells were imaged using fluorescence microscopy to assay for GFP expression. Magnetic nanoparticles were then tagged with red fluorescence and delivered to cultured ARPE-19 cells with application of a magnetic field. Uptake of particles into cells was detected using fluorescence microscopy. This procedure was repeated using Polyethylenamine (PEI)-GFP nanoparticles complexes as a standard control. Transfection efficiencies were compared using fluorescence microscopy.

Results: : Micrographs quantitating transfection efficiency of ARPE-19 cells using GFP-magnetic nanoparticle complexes will be presented and demonstrate significantly higher integration than that of PEI-RPE nanoparticles. In addition, fluorescently tagged magnetic nanoparticles showed nearly diffuse uptake by ARPE-19 cells. Finally, transfection of ARPE-19 cells using GFP-magnetic nanoparticle complexes required only 15 minutes of incubation time while exposed to magnetic field in order to achieve optimal gene delivery. In contrast, PEI-GFP nanoparticle complexes required 4 hours (as per standard protocol) of incubation time to achieve optimal delivery.

Conclusions: : The use of magnetic nanoparticles as vehicles for gene delivery in ARPE19 cells leads to high levels of nanoparticle uptake, increased transfection efficiencies and short incubation times. All of these characteristics may make magnetic nanoparticles particularly suitable to ocular gene therapy.

Keywords: gene transfer/gene therapy • retinal pigment epithelium • retina 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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