April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Pcr Detection Of Hsv-1 Dna On Diagnostic And Therapeutic Contact Lenses
Author Affiliations & Notes
  • Jean B. Kassem
    Ophthalmology, Tulane University, New Orleans, Louisiana
  • Footnotes
    Commercial Relationships  Jean B. Kassem, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1475. doi:
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      Jean B. Kassem; Pcr Detection Of Hsv-1 Dna On Diagnostic And Therapeutic Contact Lenses. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1475.

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Abstract

Purpose: : To determine whether Herpes Simplex Virus-1 can be detected on diagnostic and therapeutic contact lenses used in routine patient care, and to determine if wiping with alcohol is sufficient to remove this viral pathogen.

Methods: : Samples for testing were collected from diagnostic and therapeutic contact lenses used in routine patient care. All contact lenses were disinfected per manufacturer recommendations prior to use. 60ul of sterile, nuclease free water was used to vigorously rinse the well of the contact lens after use. A separate contact lens of the same type was used for each eye, and the sample from the right eye collected directly, while the sample from the left eye was collected after wiping the lens with a single use 70% alcohol pad for 10 seconds and air drying. The lenses were stored in separate sterile single use plastic bags if immediate collection was impossible, but no sample was collected longer than 4 hours after contact lens use. The samples were stored in sterile, labeled eppendorf tubes at -20C until the time of processing. Samples were processed using the PCR amplification method and a suitable HSV primer pair. A suitable viral DNA clone was used as a positive control to validate the PCR method. A gradient PCR program was first run with the positive control to determine the optimal program for amplification of this particular sequence. As only a small amount of the sample was used, the remaining sample material was stored at -20C for future studies.

Results: : HSV-1 DNA detected in 26 samples (n=72)HSV-1 DNA detected in 12/36 or 33% of samples from unwiped lensesHSV-1 DNA detected in 14/36 or 39% of samples from wiped lensesOdds Ratio = 0.78571, 95% C.I. (0.3,2.06)

Conclusions: : HSV-1 DNA can be isolated from diagnostic and therapeutic contact lenses used in routine patient care. Wiping with 70% alcohol for 10 seconds is insufficient for removal of genetic material from these contact lenses.

Keywords: herpes simplex virus • detection • cornea: tears/tear film/dry eye 
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