April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Effect Of Human Apolipoprotein E Genotype On The Ocular Recurrence Of Herpes Simplex Virus Type 1 Infection In Latent Mice
Author Affiliations & Notes
  • Partha S. Bhattacharjee
    Biology, Xavier University of Louisiana, New Orleans, Louisiana
  • Tashfin S. Huq
    Biology, Xavier University of Louisiana, New Orleans, Louisiana
  • Doan Thuy
    Biology, Xavier University of Louisiana, New Orleans, Louisiana
  • Kaur Jaspreet
    Biology, Xavier University of Louisiana, New Orleans, Louisiana
  • Joannie M. Ivory
    Biology, Xavier University of Louisiana, New Orleans, Louisiana
  • Valencia Potter
    Biology, Xavier University of Louisiana, New Orleans, Louisiana
  • Christina S. Simmons
    Biology, Xavier University of Louisiana, New Orleans, Louisiana
    Ophthalmology, LSUHSC, New Orleans, Louisiana
  • Christian Clement
    Ophthalmology, LSUHSC, New Orleans, Louisiana
  • Harris E. McFerrin, Jr.
    Biology, Xavier University of Louisiana, New Orleans, Louisiana
  • James M. Hill
    Ophthalmology, LSUHSC, New Orleans, Louisiana
  • Footnotes
    Commercial Relationships  Partha S. Bhattacharjee, None; Tashfin S. Huq, None; Doan Thuy, None; Kaur Jaspreet, None; Joannie M. Ivory, None; Valencia Potter, None; Christina S. Simmons, None; Christian Clement, None; Harris E. McFerrin, Jr., None; James M. Hill, None
  • Footnotes
    Support  NIH/NEI-1R21EY019144-01
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1483. doi:
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      Partha S. Bhattacharjee, Tashfin S. Huq, Doan Thuy, Kaur Jaspreet, Joannie M. Ivory, Valencia Potter, Christina S. Simmons, Christian Clement, Harris E. McFerrin, Jr., James M. Hill; Effect Of Human Apolipoprotein E Genotype On The Ocular Recurrence Of Herpes Simplex Virus Type 1 Infection In Latent Mice. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1483.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Herpetic stromal keratitis (HSK) is an eye disease caused by herpes simplex virus type 1 (HSV-1).A significant percentage of patients receiving intense therapy fail to respond. The virus can recur periodically and each recurrence can be more severe than previous ones. The recurrent HSK clinically recognized by neovascularization (new blood vessel sprouting) and opacity is chronic and more severe than acute HSK. We have reported that human apolipoprotein E (apoE) has allele-specific roles in the pathogenesis of acute HSV-1 infection in the mouse eyes (Exp. Eye. Res.87:122, 2008). Whether apoE has a similar role in the pathogenesis of recurrent HSK is unknown.

Methods: : We focused on identifying the role of human apoE genotypes in the pathogenesis of recurrent HSK in mice. Transgenic mice deleted of mouse apoE (apoE-null), knocked-in for the human apoE isoform 3 (apoE3), or apoE isoform 4 (apoE4) and their parent C57Bl/6 mice, had corneal inoculation with the high reactivating HSV-1 strain McKrae. Five weeks after acute infection, mice with clear eyes negative for virus shedding were exposed to UV irradiation (UV-B) in their right eyes. Eyes were monitored for corneal opacity, neovascularization and viral replication.

Results: : At 3 and 5 days post-UV irradiation, HSV-1 shedding from apoE4 mice was significantly higher (p < 0.05) compared to apoE3. Time onset and severity of corneal pathology (opacity and neovascularization) in apoE4 mice were also significantly higher (p < 0.05) than apoE3 mice. Compared to C57Bl/6 mice, apoE-null mice had reduced viral shedding and corneal pathology, suggesting apoE is necessary for establishment and severity of recurrent HSK in mouse eyes.

Conclusions: : This is the first report linking the isoform-specific role of human apoE in the recurrence of HSV-1 infection in mouse eyes.

Keywords: herpes simplex virus • keratitis • genetics 
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