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Derek Nankivil, Raksha Urs, Bianca Maceo, Esdras Arrieta, Fabrice Manns, Arthur Ho, Robert Augusteyn, Jean-Marie Parel; A Comparison of Biometric Properties of the Isolated Human Lens Derived from Optical Coherence Tomography, Ultrasound Biomicroscopy and Shadowphotogrammetry. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1545.
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© ARVO (1962-2015); The Authors (2016-present)
To compare calculations of equatorial diameter, central thickness, and cross-sectional area of isolated human lenses measured using three different modalities.
Optical coherence tomography (OCT) (Uhlhorn et al, Vis Res, 2008), ultrasound biomicroscopy (UBM) and shadowphotogrammetric (SP) images of 3 whole human lenses (66, 33 & 51 years, 24, 38 & 41 hours PMT) and one baboon lens (25 years, 22 hours PMT) immersed in Dulbecco’s Modified Eagle Medium at room temperature were obtained in sequence within 2 hours. Lens contours were extracted and then fit with a 10th order even Fourier series (Urs et al, Vis Res, 2010). Equatorial diameter, central thickness and cross-sectional area were calculated in terms of the fit parameters, and the uncertainty of each was estimated with an error-model analysis. Hypothesis tests were performed to determine any differences between the three measurement systems.
The average differences in the measures of diameter, thickness and cross-sectional area between the three systems were (0.7 ± 2.3)%, (0.1 ± 5.3)% and (1.0 ± 1.9)% respectively. Differences between these parameters measured with the three systems, although occasionally significant, were not consistent for all eyes, suggesting variation in lens alignment as the source of the discrepancy. SP provided more dense contours that matched the Fourier model best, giving SP better precision than OCT or UBM for the diameter, thickness and cross-sectional area (p < 0.02).
OCT, UBM and SP images provide comparable estimates of lens diameter, thickness and cross-sectional area.
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