April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Adaptive Responses After Oxidative Stress In Glutathione Depleted Lens Epithelial Cells
Author Affiliations & Notes
  • Stefan Lofgren
    St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden
  • Footnotes
    Commercial Relationships  Stefan Lofgren, None
  • Footnotes
    Support  Kronprinsessan Margaretas Arbetsnämnd för synskadade, Stiftelsen Synfrämjandets forskningsfond, Karolinska Institutet forskningsstiftelser
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1546. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Stefan Lofgren; Adaptive Responses After Oxidative Stress In Glutathione Depleted Lens Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1546.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : To investigate the response to oxidative stress in cells depleted of glutathione (GSH).

Methods: : Cultured lens epithelial cells were depleted of GSH by treatment with buthionine sulfoximine. The cells were then exposed to hydrogen peroxide. The activities of various antioxidative enzymes were followed. In addition, cell proliferation rates and levels of reactive oxygen species (ROS) were determined.

Results: : The reaction pattern among GSH-related antioxidative enzymes varied considerably. Preliminary runs indicate higher levels of ROS and almost unchanged cell proliferation rate in the control GSH-depleted cells, while peroxide treated cells exhibited a concentration dependent effect on the proliferation rate.

Conclusions: : Depletion of GSH in nonstressed cells has a minor effect on several end-points. The effects of oxidative stress in GSH-depleted cells are highly diverse, indicating a discriminative function of GSH as cofactor and scavenger.

Keywords: oxidation/oxidative or free radical damage 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.