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Daniel S. Kasprick, Tyler L. Junttila, Phillip E. Kish, Donika D. Gallina, Alon Kahana; Extracellular Matrix Structure And Remodeling During Zebrafish Lateral Rectus Regeneration. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1560.
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To study the anatomy of the extracellular matrix associated with extraocular muscles (EOMs), and investigate structural and molecular changes elicited in the process of EOM regeneration following severe injury.
Fluorescence immunohistochemistry (IHC) using confocal and epi-fluorescence microscopy was used to characterize the ECM associated with zebrafish EOMs. Antibodies against laminin, integrin, fibronectin, vinculin, and paxillin, were among the antibodies utilized. Following EOM injury, fish were sacrificed at 1-day intervals until recovery of muscle function was achieved. Sequential IHC and high-power microscopy were used to characterize the changes that occur in the ECM during the process of regeneration.
Localization patterns for each protein were distinctly organized in normal EOMs. Following lateral rectus (LR) myectomy, the residual muscle remodeled, including alterations in the structure and relative protein levels of the associated ECM. This was followed by a robust repair of the ECM along with myofibers to establish an anatomically-correct and functional LR muscle.
The composition of the extracellular matrix and distribution of key proteins is dynamic following EOM surgery and during zebrafish EOM repair and regeneration. This study lays the groundwork for further research on the relationship between ECM remodeling and EOM injury and regeneration.
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