April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Extracellular Matrix Structure And Remodeling During Zebrafish Lateral Rectus Regeneration
Author Affiliations & Notes
  • Daniel S. Kasprick
    Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, Michigan
  • Tyler L. Junttila
    Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, Michigan
  • Phillip E. Kish
    Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, Michigan
  • Donika D. Gallina
    Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, Michigan
  • Alon Kahana
    Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, Michigan
  • Footnotes
    Commercial Relationships  Daniel S. Kasprick, None; Tyler L. Junttila, None; Phillip E. Kish, None; Donika D. Gallina, None; Alon Kahana, None
  • Footnotes
    Support  NIH Grant K08EY018689 to AK; Research to Prevent Blindness Career Development Award to AK; Fight for Sight pre-doctoral award to DSK; and P30EY007003 to Dept. Ophthalmology, U. Michigan.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1560. doi:
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      Daniel S. Kasprick, Tyler L. Junttila, Phillip E. Kish, Donika D. Gallina, Alon Kahana; Extracellular Matrix Structure And Remodeling During Zebrafish Lateral Rectus Regeneration. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1560.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

To study the anatomy of the extracellular matrix associated with extraocular muscles (EOMs), and investigate structural and molecular changes elicited in the process of EOM regeneration following severe injury.

 
Methods:
 

Fluorescence immunohistochemistry (IHC) using confocal and epi-fluorescence microscopy was used to characterize the ECM associated with zebrafish EOMs. Antibodies against laminin, integrin, fibronectin, vinculin, and paxillin, were among the antibodies utilized. Following EOM injury, fish were sacrificed at 1-day intervals until recovery of muscle function was achieved. Sequential IHC and high-power microscopy were used to characterize the changes that occur in the ECM during the process of regeneration.

 
Results:
 

Localization patterns for each protein were distinctly organized in normal EOMs. Following lateral rectus (LR) myectomy, the residual muscle remodeled, including alterations in the structure and relative protein levels of the associated ECM. This was followed by a robust repair of the ECM along with myofibers to establish an anatomically-correct and functional LR muscle.

 
Conclusions:
 

The composition of the extracellular matrix and distribution of key proteins is dynamic following EOM surgery and during zebrafish EOM repair and regeneration. This study lays the groundwork for further research on the relationship between ECM remodeling and EOM injury and regeneration.  

 
Keywords: regeneration • extracellular matrix • extraocular muscles: structure 
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