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Wu Yang, Zheng Wang, Renee Paau, Mohammed Farhoud, Brian Knapp, Glenn Smits, Devin Welty; Molecular Imaging of Ocular Distribution of Bevacizumab in Rabbit Eye Following an Intravitreal Injection of 64Cu-DOTA-Bevacizumab. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1702.
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To determine the ocular distribution of Bevacizumab following intravitreal administration in rabbit eye using positron emission tomography (microPET).
Bevacizumab was conjugated with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) and tagged with 64Cu for PET imaging. 64Cu-DOTA-Bevacizumab at doses of 14.5µCi/14µg/12.5µl and 101 µCi/97µg/12.5µl were injected into the mid-vitreous of female New Zealand White rabbits. Under anesthesia the head was imaged with a microPET scanner from 5-120 minutes (dynamic imaging), and at 5, 20, 28, and 44 hour post-injection with computed tomography (microCT) for co-registration of PET and CT images. Radioactivity was quantitated to determine the in vivo biodistribution of 64Cu-DOTA- Bevacizumab.
From 5-120 minutes after injection, 64Cu-DOTA-Bevacizumab migrated from the mid-vitreous posteriorly to form an elliptical shape corresponding to the vitreous-retina interface (Figure 1). Peak accumulation on the retina surface was reached at 2 hr. Between 5 and 44 hour post-injection, 64Cu-DOTA-Bevacizumab spread to form a ball-shaped pattern around the outer surface of the eye. In contrast, 64Cu-DOTA unconjugated to Bevacizumab was rapidly eliminated from the vitreous humor within 2 hrs.
The biodistribution of 64Cu-DOTA-Bevacizumab was successfully described in the rabbit eye by non-invasive PET/CT imaging technology. Dynamic imaging revealed a distinct pattern of movement and distribution of 64Cu-DOTA-Bevacizumab towards posterior retina.Figure 1. A microPET/CT image of 64Cu-DOTA-Bevacizumab (OS) or 64Cu-DOTA (OD) distribution in NZW rabbit eye 5 hr after intravitreal injection
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