Purpose:
To assess and quantify the fluorescein angiographic macular findings of patients with macular edema pre and one month post Anti-VEGF injection, and to determine if this new concept for fluorescein angiography could correlate with OCT.
Methods:
A novel, prospective Quantative Analysis was performed on the ultra-widefield fluorescein angiograms (Optos plc.) of 20 subjects with macular edema associated with AMD, DR & RVO at baseline and one month post intra-vitreal injection of Anti-VEGF. The maximum luminosity achieved by 1st order vessels in the transit phase was used to normalize luminance values obtained throughout the fluorescein angiogram within a grid centered on the fovea and extending to a diameter of 6mm. Analysis was performed to determine if gamma normalization and luminance analysis in the macular region correlated with OCT thickness.
Results:
A value based on luminance was determined for each pixel in the macular grid, allowing color maps to be created. From the pre and post anti-VEGF fluorescein angiograms it was possible to develop luminance difference maps which were compared to OCT (Heidelberg Engineering) on the same patients.
Conclusions:
Assessing perfusion and/or ischemia in the macula by quantification of normalised luminance appears to be a valuable clinical adjunct in determining the efficacy of anti-VEGF therapy in patients with macular edema.
Keywords: imaging/image analysis: clinical • edema • macula/fovea