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Serge G. Rosolen, Sylvie Lavillegrand, Mei-Lyn Riviere, Manuel Simonutti, Jose-Alain Sahel, Serge Picaud, Jean-Francois LeGargasson; Suitability Of A Novel Human Multimodal Slit-lamp Sd-oct To Image Animal Eyes. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1727.
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© ARVO (1962-2015); The Authors (2016-present)
To obtain images of the anterior and the posterior segments using a novel human multimodal slit-lamp SD-OCT for experimental, toxicological and clinical investigations in small (rodents) and large animal species (dogs, cats, rabbits and minipigs).
3 albino rats, 3 pigmented rats, 5 dogs, 5 cats, 4 NZW rabbits and 4 minipigs were assessed. All animals were sedated with medetomidine (0.1 mg/kg) except for rats which were awake. Pupil dilation was performed in order to obtain eye fundus images. Images were obtained using a Topcon slit-lamp SL-D7 equiped with a SD-OCT (axial resolution: 8-9 µm; lateral resolution:< 20 µm; scanning range: 2-12 mm anterior and posterior). Retinal thickness measurements were performed in periphery, area centralis and near the papilla.
For all animals, "en face" images and slit images of eye structures and OCT images of the cornea and the retina were easily obtained without any additional optical device. OCT images enabled identification of the corneal layers (epithelium, stroma, endothelium) and the central and peripheral retinal layers (choriocapillaris and RPE, photoreceptors layer, nerve fiber layer) in all animal species. The retinal thickness measurements near the area centralis and near the papilla were 303.3 µm (+/-23.6) and 374.0 µm (+/-30.7) for cats, 284.3 µm (+/-43.2) and 349.4 µm (+/-58.6) for dogs and 326.0 µm (+/-31.7) and 441.1 µm (+/-58.9) for minipgs. The retinal thickness measurements in periphery were 126.8 µm (+/-44.7), 211.7 µm (20.7) and 250.1 µm (+/-24.3) for rabbits, albino and pigmented rats, respectively, values that are compatible with existing literature.
Images of anterior and posterior segments were easily obtained without any additional optical device in small and large size animal species and under sedation or awake. This non invasive procedure is useful for experimental, toxicological and clinical assessments as well. In vivo measurements of retinal structures indicated than in animals species tested the peripheral retina is thinner than central retina with a prominent thickening in peripapillar area. OCT measurements of retinal structures involved in either spontaneous or induced corneal and retinal diseases combined with a slit-lamp device could find applications in pharmacological and toxicological investigations and for clinical applications in veterinary medicine as well.
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