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Sepideh Hariri, Alireza Akhlagh Moayed, Eun Sun Song, Kostadinka K. Bizheva; Quantification of the Retina Layers Reflectivity and Thickness in an In-vivo Rat model of Outer Retina Degeneration with Ultrahigh Resolution Optical Coherence Tomography. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1731.
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© ARVO (1962-2015); The Authors (2016-present)
To visualize in 3D and quantify in-vivo the early changes in the layer reflectivity and structure in a rat retina degeneration model induced by a retinal toxin, sodium iodate, by use of a high speed, ultrahigh resolution optical coherence tomography (UHROCT) system.
A research-grade, high speed, UHROCT system, was developed for non-invasive rodent retina imaging. The system provides 3µm axial and ~5µm lateral resolution in the rat retina. A semi-automatic segmentation algorithm was applied to the UHROCT tomograms for evaluation of the reflectivity (intensity) and thickness of individual retinal layers. Outer retinal damage was induced by intravenous injection of sodium iodate in adult, female Long Evans rats. Volumetric UHROCT tomograms were acquired in a longitudinal study at baseline, 6h, 12h and days 1, 3, 7 post injection.
2D and 3D OCT images of healthy rodent retinas showed clear visualization of all retinal layers, RPE, and choroidal vasculature. Preliminary results from UHROCT tomograms acquired post sodium iodate injection showed progressive loss of the photoreceptor layer by initial change in the intensity and thickness and eventually loss of the outer segment(OS)/RPE junction during the first 24 hours. These changes were followed by progressive intensity change and loss of the remaining OS section (low reflective band) and the IS/OS junction by day7. Loss of the ELM and changes in the ONL along with swelling of the retina were also observed and quantified at all time-points of the longitudinal study.
The use of fast UHROCT technology permitted accurate, spatially dependent measurements of the progressive changes in the optical reflectivity and layer thickness of individual retinal layers in a drug-induced rodent morel of outer retina degeneration.Results from this study could potentially help better understanding and diagnosis of blinding degenerative diseases.
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