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Concetta Triglia, Derek Nankivil, Elizabeth Fout-Caraza, William Lee, Cornelius Rowaan, Carolina De Freitas, Sonia H. Yoo, Jean-Marie A. Parel; Descemet-Stripping Automated Endothelial Keratoplasty Corneal Sections Measured by Optical Coherence Tomography and Ultrasound Pachymetry: A Comparison. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1739.
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To compare human donor sectioned corneal thickness measurements made by non-contact optical coherence tomography (OCT) and contact ultrasound (US) pachymetry.
Post-mortem corneas were dissected from 10 donor globes - and were preserved in Optisol-GS (B&L, Rochester, NY). The corneas were then mounted on a microkeratome system (Moria, France) and were sectioned using a 300 micron head (119 to 182 hours post harvest time, 152 ± 22 hours). After resection and while still mounted on the artificial anterior chamber, the central thickness of each cornea was measured using a conventional US pachymeter (Advent Pachymeter, Accutome, PA). Each Descemet-Stripping Automated Endothelial Keratoplasty (DSAEK) section was then transferred into a vial containing Optisol-GS at room temperature and the vial was positioned in front of the Visante OCT using a special adaptor (Abeamed, Miami FL) and the central thickness of the DSAEK section was measured. The measured thicknesses obtained with OCT and US were compared.
The mean US and OCT measured thickness was 226 ± 75 µm and 247 ± 76 µm respectively. The difference in measured sectioned thickness (OCT - US) was 21 ± 18 µm (p < 0.007). The two systems provide thicknesses that are highly correlated (p < 0.0001). After more than 100 hours of post harvest storage, linear regression of the DSAEK section central thickness indicates a significant correlation with post-mortem time (p < 0.003), increasing at a rate of 3 ± 2 µm per hour.
Non-contact Visante OCT yields slightly larger measurements of DSAEK donor thickness than US pachymetry, which required pressure on the tissue to obtain a measurement; however, the two systems are significantly correlated. OCT cannot contaminate tissues and therefore has this advantage over US pachymetry.
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