April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Genetic Confirmation Of Target-independent Suppression Of Choroidal Neovascularization By siRNAs Via TLR3
Author Affiliations & Notes
  • Yoshio Hirano
    Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky
  • Hiroki Kaneko
    Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky
  • Judit Baffi
    Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky
  • Sami Dridi
    Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky
  • Valeria Tarallo
    Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky
  • Sasha Bogdanovich
    Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky
  • Bradley Gelfand
    Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky
  • Mark E. Kleinman
    Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky
  • Royce Mohan
    Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky
  • Jayakrishna Ambati
    Ophthalmology and Visual Sciences, University of Kentucky, Lexington, Kentucky
  • Footnotes
    Commercial Relationships  Yoshio Hirano, None; Hiroki Kaneko, None; Judit Baffi, None; Sami Dridi, None; Valeria Tarallo, None; Sasha Bogdanovich, None; Bradley Gelfand, None; Mark E. Kleinman, None; Royce Mohan, None; Jayakrishna Ambati, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1766. doi:
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      Yoshio Hirano, Hiroki Kaneko, Judit Baffi, Sami Dridi, Valeria Tarallo, Sasha Bogdanovich, Bradley Gelfand, Mark E. Kleinman, Royce Mohan, Jayakrishna Ambati; Genetic Confirmation Of Target-independent Suppression Of Choroidal Neovascularization By siRNAs Via TLR3. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1766.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We previously reported that non-targeting, cell-impermeant siRNAs can suppress neovascularization in various models of angiogenesis via generic activation of toll-like receptor 3 (TLR3) without triggering RNA interference. In this study, we examined whether siRNAs targeting Vimentin or Timp2 could suppress laser-induced choroidal neovascularization (CNV) in Vim-/-, Timp2-/-, and Tlr3-/- mice.

Methods: : The CNV responses of Vim-/-, Timp2-/-, and Tlr3-/- mice treated with intravitreous administration of siRNA-Vimentin or siRNA-Timp2 were quantified by volumetric analysis.

Results: : siRNA-Vimentin and siRNA-Timp2 suppressed CNV in mice deficient in these genes, respectively. However, CNV was not suppressed by these siRNAs in Tlr3-/- mice.

Conclusions: : These results provide genetic corroboration of target-independent suppression of CNV by siRNAs via TLR3.

Keywords: age-related macular degeneration 
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