April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
A 21-Day Comparative Study of Laser Induced Choroidal Neovascularisation in Long Evans (LE) and Brown-Norway (BN) Rats
Author Affiliations & Notes
  • Genevieve Glazier
    Charles River, Senneville, Quebec, Canada
  • Mark Vezina
    Charles River, Senneville, Quebec, Canada
  • Martin Bussieres
    V&O Services, St. Lazare, Quebec, Canada
  • Marie-Pier Gagnon
    Charles River, Senneville, Quebec, Canada
  • Tanya Halle
    Charles River, Senneville, Quebec, Canada
  • Tim Bryant
    Charles River, Senneville, Quebec, Canada
  • Footnotes
    Commercial Relationships  Genevieve Glazier, Charles River (E); Mark Vezina, Charles River (E); Martin Bussieres, Charles River (C); Marie-Pier Gagnon, Charles River (E); Tanya Halle, Charles River (E); Tim Bryant, Charles River (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1800. doi:
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      Genevieve Glazier, Mark Vezina, Martin Bussieres, Marie-Pier Gagnon, Tanya Halle, Tim Bryant; A 21-Day Comparative Study of Laser Induced Choroidal Neovascularisation in Long Evans (LE) and Brown-Norway (BN) Rats. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1800.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To compare strain and sex differences in a rat model of laser-induced choroidal neovascularization (CNV) in the presence or absence of rat-specific anti-VEGF antibody (ARVA).

Methods: : Four bilateral laser ruptures of Bruch’s membrane using a diode laser were created on Day 1 concurrent with intravitreal injection of ARVA (2 µL, 4 µg/eye) or phosphate buffered saline (PBS, 2 µL), followed by a second dose on Day 13. Fluorescein angiograms (FA) were obtained on Days 7, 14 and 21 using a CSLO. Individual laser spots were evaluated for fluorescein leakage semiquantatively on a scale of 0-4.

Results: : No sex differences in the incidence of clinically relevant lesions (Grade 3 or 4) were noted for either strain in eyes treated with PBS. Overall mean FA scores and incidence of Grade 3 and 4 lesions were higher in BN rats receiving PBS when compared to LE rats, suggesting increased CNV in BN rats. ARVA appeared to decrease CNV in LE males on Days 7 and 14 and on Day 7 only for LE females. CNV in male BN rats given ARVA was decreased on Day 14 only. CNV in female BN rats was unaffected or increased when compared to PBS on most occasions.

Conclusions: : While BN rats administered PBS developed more severe CNV than LE rats, the LE rats appeared to be more sensitive to intravitreal ARVA. The ARVA results were variable and more apparent in the males of both strains. Inflammatory response (not evaluated) to the murine-derived ARVA may have played a role in this variability and/or the ARVA dose of 4 µg/eye given twice intravitreally, two weeks apart may not have been optimal to decrease neovascularization.

Keywords: choroid: neovascularization • laser • age-related macular degeneration 

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