April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Genetic Ablation Of Rod Cyclic-nucleotide Gated (cng) Channels Rescues Rd1 Mouse Retinal Degeneration And Preserves Cone Photoreceptor Function
Author Affiliations & Notes
  • Francois Paquet-Durand
    Experimental Ophthalmology,
    Institute for Ophthalmic Research, Tuebingen, Germany
  • Susanne Beck
    Ocular Neurodegeneration,
    Institute for Ophthalmic Research, Tuebingen, Germany
  • Stylianos Michalakis
    Ludwig-Maximilians-Universität München, Munich, Germany
  • Tobias Goldmann
    Johannes Gutenberg University Mainz, Mainz, Germany
  • Gesine Huber
    Ocular Neurodegeneration,
    Institute for Ophthalmic Research, Tuebingen, Germany
  • Regine Mühlfriedel
    Experimental Ophthalmology,
    Institute for Ophthalmic Research, Tuebingen, Germany
  • Elvir Becirovic
    Ludwig-Maximilians-Universität München, Munich, Germany
  • Uwe Wolfrum
    Johannes Gutenberg University Mainz, Mainz, Germany
  • Naoyuki Tanimoto
    Ocular Neurodegeneration,
    Institute for Ophthalmic Research, Tuebingen, Germany
  • Mathias Seeliger
    Ocular Neurodegeneration,
    Institute for Ophthalmic Research, Tuebingen, Germany
  • Footnotes
    Commercial Relationships  Francois Paquet-Durand, None; Susanne Beck, None; Stylianos Michalakis, None; Tobias Goldmann, None; Gesine Huber, None; Regine Mühlfriedel, None; Elvir Becirovic, None; Uwe Wolfrum, None; Naoyuki Tanimoto, None; Mathias Seeliger, None
  • Footnotes
    Support  DFG: Se837/5-2, Se837/6-1, Se837/7-1, Pa1751/1-1; BMBF: 0314106; EU: HEALTH-F2-2008-200234, NEUROTRAIN-MEST-CT-2005-020235; CIN: pool project 2009-20; Kerstan Foundation, Faun Foundation
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1806. doi:
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      Francois Paquet-Durand, Susanne Beck, Stylianos Michalakis, Tobias Goldmann, Gesine Huber, Regine Mühlfriedel, Elvir Becirovic, Uwe Wolfrum, Naoyuki Tanimoto, Mathias Seeliger; Genetic Ablation Of Rod Cyclic-nucleotide Gated (cng) Channels Rescues Rd1 Mouse Retinal Degeneration And Preserves Cone Photoreceptor Function. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1806.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract 
 
Purpose:
 

The rd1 mouse is one of the most commonly studied animal models for retinitis pigmentosa (RP), a severe and frequently blinding human retinal degeneration. In several decades of research, the link between mutation-induced increase in photoreceptor cGMP levels and the extremely rapid cell death has remained poorly understood. Here, we set out to study the role of cyclic-nucleotide-gated (CNG) channels in the degenerative process, by crossing CNGb1-/- animals with rd1 mutant mice.

 
Methods:
 

Single mutant rd1 and CNGb1 animals as well as double mutant CNGb1-/- x rd1 animals were analysed using in vivo optic coherence tomography and scanning laser ophthalmoscopy imaging techniques, combined with electroretinographic functional testing. Ex vivo analysis included assessment of rod and cone photoreceptor markers, degenerative markers (calpain activity, TUNEL), cGMP contents, and electron microscopic ultrastructural analysis of rod outer segments.

 
Results:
 

In Cngb1-/- x rd1 double mutants devoid of regular CNG channels, cGMP levels were strongly elevated, but rod photoreceptor viability and outer segment morphology were greatly improved. Importantly, cone photoreceptors, the basis for high-resolution daylight and colour vision, survived and remained functional for extended periods of time.

 
Conclusions:
 

We demonstrated the major importance of CNG channels for rod photoreceptor loss supporting the hypothesis of deleterious calcium (Ca2+)-influx as a cause of rapid rod cell death. Furthermore, our results suggest that targeting rod CNG channels, rather than general Ca2+-channel blockade, is a promising symptomatic approach to treat otherwise incurable forms of cGMP-related RP.

 
Keywords: retinal degenerations: hereditary • photoreceptors • second messengers 
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