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Xin Wang, Xiaofen Mo, Tiande Shou, Yan Wang, Yuan Fang; Neuroprotective Effect of Transcorneal Electrical Stimulation on Ischemic Damage in the Rat Retina. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1866.
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To investigate whether transcorneal electrical stimulation (TES) has a neuroprotective effect on retinal neurons after ischemic insults and the underlying mechamism.
Adult female Sprague-Dawley (SD) rats received TES after ocular ischemia was induced by elevating the intraocular pressure to 120 mmHg for 60 minutes. Retinal ganglion cells (RGCs) were labeled retrogradely 7 days before ischemia and were quantified 7 and 14 days later. At the same time points, retinal function was assessed by scotopic electroretinography (ERG), combined with retinal histological analysis. The glutamine synthetase (GS) immunoreactivity was compared between ischemic retinas with and without TES under identical confocal laser microscope conditions. The immunohistochemical indications were confirmed by Western blot analysis.
Higher mean density of RGCs was quantified in TES treated retinas compared to retinas without TES on days 7 and 14 after ischemia. Similarly, histological analysis showed that TES better preserved the mean thickness of separate retinal layers. ERG studies indicated that by undergoing TES treatment, the b-wave amplitude was also significantly preserved on day 7 after ischemia and recovered robustly on day 14. Immunohistochemical and Western blot analysis both revealed that GS levels remarkably increased after TES and lasted for at least 7 days.
TES can protect retinal neurons against ischemic insults, probably related to increasing levels of GS localized in Müller cells. These findings suggest a new approach to the clinical handling of ocular ischemic diseases.
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