April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Neuroprotective Effect of Transcorneal Electrical Stimulation on Ischemic Damage in the Rat Retina
Author Affiliations & Notes
  • Xin Wang
    Ophthalmology and Vision Science, Eye and ENT Hospital,Fudan University, Shanghai, China
  • Xiaofen Mo
    Ophthalmology and Vision Science, Eye and ENT Hospital,Fudan University, Shanghai, China
    Brain Science and State Key Laboratory of Medical Neurobiology,Fudan University, Shanghai, China
  • Tiande Shou
    Ophthalmology and Vision Science, Eye and ENT Hospital,Fudan University, Shanghai, China
    vision Research Lab, Center for Brain Science Research,School of Life Sciences,Fudan University, Shanghai, China
  • Yan Wang
    Ophthalmology and Vision Science, Eye and ENT Hospital,Fudan University, Shanghai, China
  • Yuan Fang
    Ophthalmology and Vision Science, Eye and ENT Hospital,Fudan University, Shanghai, China
  • Footnotes
    Commercial Relationships  Xin Wang, None; Xiaofen Mo, None; Tiande Shou, None; Yan Wang, None; Yuan Fang, None
  • Footnotes
    Support  Program for New Century Excellent Talents in University by the Ministry of Education (Grant No. NCET-05-0370); the National Basic Research Program of China (Grant No. 2007CB512205)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1866. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Xin Wang, Xiaofen Mo, Tiande Shou, Yan Wang, Yuan Fang; Neuroprotective Effect of Transcorneal Electrical Stimulation on Ischemic Damage in the Rat Retina. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1866.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose:
 

To investigate whether transcorneal electrical stimulation (TES) has a neuroprotective effect on retinal neurons after ischemic insults and the underlying mechamism.

 
Methods:
 

Adult female Sprague-Dawley (SD) rats received TES after ocular ischemia was induced by elevating the intraocular pressure to 120 mmHg for 60 minutes. Retinal ganglion cells (RGCs) were labeled retrogradely 7 days before ischemia and were quantified 7 and 14 days later. At the same time points, retinal function was assessed by scotopic electroretinography (ERG), combined with retinal histological analysis. The glutamine synthetase (GS) immunoreactivity was compared between ischemic retinas with and without TES under identical confocal laser microscope conditions. The immunohistochemical indications were confirmed by Western blot analysis.

 
Results:
 

Higher mean density of RGCs was quantified in TES treated retinas compared to retinas without TES on days 7 and 14 after ischemia. Similarly, histological analysis showed that TES better preserved the mean thickness of separate retinal layers. ERG studies indicated that by undergoing TES treatment, the b-wave amplitude was also significantly preserved on day 7 after ischemia and recovered robustly on day 14. Immunohistochemical and Western blot analysis both revealed that GS levels remarkably increased after TES and lasted for at least 7 days.

 
Conclusions:
 

TES can protect retinal neurons against ischemic insults, probably related to increasing levels of GS localized in Müller cells. These findings suggest a new approach to the clinical handling of ocular ischemic diseases.  

 
Keywords: neuroprotection • ischemia • protective mechanisms 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×