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Jose-Manuel Ramirez, Rosa De Hoz, Juan J. Salazar, Ana I. Ramirez, Blanca Rojas, Beatriz I. Gallego, Manuel Salinas-Navarro, Arturo Ortin-Martinez, Maria P. Villegas-Perez, Alberto Trivino; IOP Induces Changes In The GFAP-Labelled Retinal Area But Not In Astrocyte Number In Mice Retina Contralateral To Experimental Glaucoma. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2025.
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To analyse the effects of laser-induced ocular hypertension (OHT) in the astrocytes of contralateral eyes two weeks after lasering.
Adult Swiss mice were divided into two groups: naïve (n=6) and lasered (n=6). Retinal whole-mounts were immunostained with antibodies against GFAP. The GFAP-labelled retinal area (GFAP-RA) and the number of astrocytes were quantified.
In comparison with naïve: i) astrocytes were more robust in contralateral eyes and had a less intense GFAP immunoreaction (GFAP-IR) with fewer secondary processes in OHT-eyes; ii) GFAP-RA increased in contralateral eyes (p<0.032) and decreased in OHT-eyes (p<0.001); iii) there were no differences in astrocyte number in contralateral or OHT-eyes. However, 57.02% (5523 of 9686) of the astrocytes in OHT-eyes had weaker GFAP-IR and fewer secondary processes than did naïve eyes.
Two weeks of laser-induced OHT caused changesin the GFAP-labelled retinal area but not in astrocyte number in both contralateral and OHT-eyes. On the basis of the astroglial changes detected in the present work, the use of the contralateral eye as an internal control in experimental model unilateral OHT should be reconsidered.
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