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Blanca Rojas, Juan J. Salazar, Ana I. Ramirez, Rosa De Hoz, Beatriz I. Gallego, Manuel Salinas-Navarro, Luis Alarcon-Martinez, Manuel Vidal-Sanz, Alberto Trivino, Jose-Manuel Ramirez; Microglial Population In Contralateral Mice Retina To Experimental Glaucoma: A Qualitative And Quantitative Study. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2026.
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© ARVO (1962-2015); The Authors (2016-present)
To analyse the qualitative and quantitative changes of laser-induced ocular hypertension (OHT) in microglial population of eyes with OHT (OHT-eyes) and contralateral eyes two weeks after lasering.
Adult albino Swiss mice were divided in two groups: naive; (age-matched control; n=6) and lasered (n= 6). Retinal whole-mounts were immunostained with anti-Iba1, and the morphology, density, and distribution of Iba1+ microglial cells were analysed.
In the three study groups, Iba1+ microglias were observed throughout the retina, distributed in the subretinal space (SS), outer plexiform layer (OPL), inner plexiform layer (IPL), ganglion-cell layer (GCL) and nerve-fibre layer (NFL). In naïve eyes, Iba1+ microglias in the OPL and IPL exhibited a branched morphology emanating from small cell bodies. By contrast, Iba1+ microglias in the SS had rounded bodies with fewer and shorter processes; in the NFL and in the GCL, Iba1+ microglias were less ramified and were distributed parallel to axons of the retinal ganglion cells. Iba1+ cells in contralateral and OHT-eyes exhibited morphological features that differed from naïve:i) Iba1+ microglial bodies were more robust; ii) Iba1+ microglial processes were thicker and more branched in contralateral eyes and thicker and retracted in OHT-eyes. The number of Iba1+ microglias in OHT-eyes and contralateral untreated eyes increased in comparison to naïve (p< 0.001 and p< 0.041 respectively).
Two weeks of laser induced-OHT triggered microglial retinal changes in the contralateral untreated and OHT-eyes. The microglial behaviour in contralateral untreated eyes, lead us to suggest that the use of contralateral eyes as internal controls in experimental unilateral OHT, should be reconsidered.
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