Purchase this article with an account.
Alireza Akhlagh Moayed, Sepideh Hariri, Vivian Choh, Kostadinka K. Bizheva; In Vivo Measurement And Correlation Of Visually Evoked Retinal And Pupil Responses Of Chicken With Functional Optical Coherence Tomography. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2192.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To measures in vivo visually evoked intrinsic optical signals (IOSs) from individual layers of the chicken retina using functional optical coherence tomography (fOCT) and correlate their time course with the pupil response to visual stimuli of different colour, intensity and duration.
A high speed fOCT system was used to measure in vivo visually-evoked IOSs from individual layers of the chicken retina and pupil dynamics in response to optical stimuli of different color, duration and intensity. The 1060 nm fOCT system provided 3 µm axial and ~ 5 µm resolutions in the chicken retina and pupil, at a 22 µs line scan rate and ~7 ms time resolution for volumetric integration of the retinal IOSs. The fOCT system was interfaced with a full field ERG system (Diagnosys LLC), and was used for generation of the visual stimuli and collection of ERG traces simultaneously with the IOS recordings. IOS, ERG and pupil dynamics recordings were acquired from healthy White Leghorn (Gallus gallus domesticus) chicken.
fOCT recordings from the inner retinal layers showed positive IOSs (increase in tissue reflectivity) peaking at ~33 ms and negative IOSs (decrease in the reflectivity), peaking at 67 ms to 73 ms post stimulus on-set. IOSs measured from the OS and RPE showed a strong negative peak at 34 ms and a positive one at 67 ms post stimulus on-set. Pupil constriction was observed as early as ~50 ms post stimulus on-set, peaking at ~150 ms with a subsequent slow dilation that continued for more than 1s post stimulus on-set.
Reproducible visually evoked IOSs were measured from all retinal layers in an in vivo chicken model with a combined fOCT+ERG system. Direct correlation was established between the time courses of the measured IOS, ERG and pupil dynamics. These results show the ability of fOCT technology for probing non-invasively light-induced physiological processes in the animal retina.
This PDF is available to Subscribers Only