March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Retinal Hemodynamic Response to Hyperoxia
Author Affiliations & Notes
  • Margaret M. O' Halloran
    Applied Optics Group, School of Physics, National University of Ireland, Galway, Galway, Ireland
  • Chris Dainty
    Applied Optics Group, School of Physics, National University of Ireland, Galway, Galway, Ireland
  • Eamonn O' Donoghue
    Department of Ophthalmology, University College Hospital Galway, Galway, Ireland
  • Footnotes
    Commercial Relationships  Margaret M. O' Halloran, None; Chris Dainty, None; Eamonn O' Donoghue, None
  • Footnotes
    Support  PTRLI-4 and SFI grant 07/IN1/1906
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 2202. doi:
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      Margaret M. O' Halloran, Chris Dainty, Eamonn O' Donoghue; Retinal Hemodynamic Response to Hyperoxia. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2202.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To improve the accuracy in the determination of the vascular reactivity of normal, healthy eyes during hyperoxic provocation using a high resolution imaging modality - a confocal scanning laser ophthalmoscope.

Methods: : Imaging is performed with a manufacturer modified HRT classic. It has been modified to enable imaging of the retina at a 10, 2, or 1 degree field of view (FOV). It acquires a sequence of 32 frames at a single focal plane to obtain a video of the vasculature in approximately 1.5 s. A number of sequences were obtained, at the 2 degree FOV, of a selected vessel segment before and during the administration of 100% oxygen via a non-rebreather mask. Each sequence was then registered and averaged. The vessel diameter before and during hyperoxic provocation was determined using vessel tracking and edge detection techniques applied to the average image, and the magnitude of vasoconstriction was subsequently calculated.

Results: : 8 eyes of 7 normal subjects were imaged. Arterioles approximately 1 disc diameter from the optic nerve head were imaged before and during hyperoxia. A constriction of the vessels was observed in all cases, with a mean % constriction of 8.1 ± 2.0 %. To investigate the repeatability, the same vessel segment in one subject was measured during 3 different imaging sessions. A mean % constriction of 8.32 ± 0.2 % was obtained.

Conclusions: : To the best of our knowledge, this is the first application of a high resolution imaging modality to the assessment of retinal vascular reactivity. This promises to improve the accuracy and reproducibility of the assessment of reactivity.

Keywords: blood supply • imaging/image analysis: non-clinical • image processing 

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