April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Investigation Of Pax6 Function In Mouse Ocular Surface Mesenchyme Using A Tissue Specific Mouse Model, Wnt1cre+/Pax6flox/RosamTmG
Author Affiliations & Notes
  • Minh-Thanh T. Nguyen
    Ophthalmology, University of Cincinnati, Cincinnati, Ohio
  • Chia-yang Liu
    Ophthalmology, University of Cincinnati, Cincinnati, Ohio
  • Ruth Ashery-Padan
    Human Molecular Genetics and Biochemistry, Tel Aviv University, Tel Aviv, Israel
  • Winston W. Kao
    Ophthalmology, University of Cincinnati, Cincinnati, Ohio
  • Footnotes
    Commercial Relationships  Minh-Thanh T. Nguyen, None; Chia-yang Liu, None; Ruth Ashery-Padan, None; Winston W. Kao, None
  • Footnotes
    Support  NIH EY 013755, Research to Prevent Blindness, Inc. and Ohio Lions Eye Research Foundation
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1925. doi:
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      Minh-Thanh T. Nguyen, Chia-yang Liu, Ruth Ashery-Padan, Winston W. Kao; Investigation Of Pax6 Function In Mouse Ocular Surface Mesenchyme Using A Tissue Specific Mouse Model, Wnt1cre+/Pax6flox/RosamTmG. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1925.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine the role of Pax6 in the periocular mesenchymal cells, which contribute to corneal stromal, endothelial, trabecular meshwork, and iris and ciliary body stromal development.

Methods: : We crossed Wnt1Cre with Pax6flox/flox and ROSAmTmG/mTmG transgenic mice to ablate Pax6 in periocular mesenchymal cells in triple transgenic animals, Wnt1cre/Pax6flox/+/ROSAmTmG/+ and Wnt1cre/Pax6flox/flox/ROSAmTmG/+. Pax6 was conditionally deleted in Wnt1Cre+ cells. The effect of heterozygous (Pax6flox/+) or homozygous (Pax6flox/flox) Pax6 deletion in the Wnt1Cre+ cells was examined by stereomicroscope and by immunohistochemistry. All reported research was conducted in compliance with the ARVO statement for the Use of Animals in Ophthalmic and Vision Research.

Results: : About 50% of Wnt1cre+/Pax6flox/+/ROSAmTmG mice developed corneal haze and growth of blood and lymphatic vessels from the limbus into the central cornea by 12 weeks. Wnt1cre+/Pax6flox/flox/ROSAmTmG mice died from hydrocephaly starting around 4 to 6 weeks post-natal. The dome-shaped head is already recognizable at day 12 post-natal. H&E staining of Wnt1cre+/Pax6flox/flox/ROSAmTmG 4 weeks-old mice indicated that the iridocorneal angle, the iris and ciliary body (CB) stroma, the corneal stroma and the endothelial layers are not affected. Immunofluorescent staining with anti-LYVE1 antibody showed that the lymphatic vessels developed beyond the boundary between limbus and peripheral cornea.

Conclusions: : The Wnt1cre+/Pax6flox/ROSAmTmG transgenic line allows us to study the functional role of Pax6 in the periocular mesenchymal cells. The ablation of Pax6 in Wnt1Cre+ cells do not lead to iris and CB stromal, corneal stromal, nor corneal endothelial defects. However, it appeared to affect corneal lymphangiogenesis/angiogenesis.

Keywords: cornea: basic science • cornea: stroma and keratocytes • genetics 
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