April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Azithromycin (Azyter®) Reduces Inflammation In A Rat Model Of Acute Conjunctivitis
Author Affiliations & Notes
  • Patricia Fernandez
    Experimental Ophthalmology Laboratory, Universidad de Navarra, Pamplona Navarra, Spain
  • Sergio Recalde
    Experimental Ophthalmology Laboratory, Universidad de Navarra, Pamplona Navarra, Spain
  • Maite Moreno-Orduña
    Experimental Ophthalmology Laboratory, Universidad de Navarra, Pamplona Navarra, Spain
  • Laura García-García
    Experimental Ophthalmology Laboratory, Universidad de Navarra, Pamplona Navarra, Spain
  • Vanessa Fernández-García
    Experimental Ophthalmology Laboratory, Universidad de Navarra, Pamplona Navarra, Spain
  • Alfredo García-Layana
    Experimental Ophthalmology Laboratory, Universidad de Navarra, Pamplona Navarra, Spain
    Ophthalmology, Clinica Universidad de Navarra, Pamplona, Navarra, Spain
  • Footnotes
    Commercial Relationships  Patricia Fernandez, None; Sergio Recalde, None; Maite Moreno-Orduña, None; Laura García-García, None; Vanessa Fernández-García, None; Alfredo García-Layana, None
  • Footnotes
    Support  Supported by Thea Laboratoires and PFR received a grant from Fundación Gangoiti.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1931. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Patricia Fernandez, Sergio Recalde, Maite Moreno-Orduña, Laura García-García, Vanessa Fernández-García, Alfredo García-Layana; Azithromycin (Azyter®) Reduces Inflammation In A Rat Model Of Acute Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1931.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Macrolide antibiotics are known to have a variety of anti-inflammatory effects in addition to antimicrobial activity, but the mechanisms are still unclear. We investigated the effect of azithromycin on inflammatory molecules in lipopolysaccharide (LPS)-induced rat conjunctivitis.

Methods: : Twenty-four wistar rats were divided into 2 groups receiving topical ocular azithromycin (AZ; 15 mg/g) or vehicle (V). In total, six doses (25 µl) were administered as one dose twice a day for three days prior to subconjunctival LPS injection (3 mg/ml). Before sacrifice, mucus secretion, conjunctival and palpebral edema and redness were evaluated. Real time PCR was used to determine gene expression for IL-6, COX-2, TNF-α, MMP-2 and MMP-9. IL-6 and TNF-α tissue levels were determined by ELISA, NFΚB by western-blot and MMP-9 and MMP-2 activity by gelatin zymogram. Four eyes per group were processed for histology and subsequent Masson’s Trichrome staining. T-student analysis or Wilcoxon test for independent samples were applied (SPSS v.15.0). Data assist v 2.0 was used for gene expression quantitative analysis.

Results: : AZ group showed a significant reduction of all clinical signs (P<0.05) when compared with V group. Furthermore, IL-6 (P=0.043) and NFΚB protein expression (P=0.004) and MMP-2 activity (P=0.018) in conjunctival homogenates were significantly reduced compared with vehicle administered animals. MMP-2 gene expression showed a tendency to significantly reduce in AZ group (P=0.063). Moreover, mucus secretion by Goblet cells was also reduced in AZ group.

Conclusions: : These results suggest that azithromycin administration ameliorate LPS-induced inflammation effects in a rat model of acute conjunctivitis.

Keywords: conjunctivitis 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×