Abstract
Purpose: :
To study efficiency of the introstromal introductions of the autologic mononuclear blood cells in treatment of the induced damage of a cornea.
Methods: :
A series of experiments on 20 rabbits of breed the Chinchilla. Corneal epithelium was removed out totally and mechanical damage of stroma and endothelium was executed. The animals of the 1st group besides antibacterial and metabolic remedies was injected autological mononuclear blood cells into the stroma. The cells allocated by the method fractionating centrifugation on a density gradient. In the 2nd animal group traditional pharmacotherapy was given. The general duration of experiment was 21 days. During experiment external examination, biomicroscopy, fluorescent test, photoregistration were spent. The material fence was made for 3, 7, 14, 21 days of the experiment.
Results: :
For the 3d day of experiment corneal epithelium of the 1st group was presented by 5-6 layers of flat cells, in the stroma was observed diffuse mononuclear infiltration, bunches of collagen fibers settled disordered down. In the 2nd group corneal epithelium was presented by hydropical dystrophic epitheliocites. The stroma was edematous, collagenic fibers were friable located. At the limbus was found out dense polymorphic nuclear infiltration. Corneal epithelium was desquamated. At the 7th day, in animals of the 1st group, the course of collagen fibers in corneal stroma became ordered. In limbic area was found out diffuse congestion of mononuclear cells and the individual expanded vessels of. The endothelium remained on all extent. In animals of the 2nd group, the nidal fibrin deposits was applying on the cornea, diffuse lymphocytic infiltration and neovascularisation were observed in the stroma. At the 14th day at animals of the 1st group corneal epithelium had a normal structure. The stroma contained strictly located collagenic fibres. In the 2nd group in back 1/3 own substance of the cornea was homogeneous destruction of the corneal stroma. At the 21st day of the experimant, the corneal epithelium in animals of the 1st group had the normal structure. In the 2nd group the Boumen’s membrane was a little thickened. At the 2/3 parts was marked active formation of collagenic fibres, in back 1/3 of stromal layers - the hypostasis and homogenization remained.
Conclusions: :
The intrastromal introduction of the autologic mononuclear cells at cases of cornea damage stimulates reparative regeneration, promoting fast healing and thus interfering scarring and neovascularisation of the cornea.
Keywords: degenerations/dystrophies • regeneration • wound healing